Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Ronny Helland"'
Autor:
Scott Mazurkewich, Ronny Helland, Alasdair Mackenzie, Vincent G. H. Eijsink, Phillip B. Pope, Gisela Brändén, Johan Larsbrink
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Abstract Chitin is one of the most abundant renewable organic materials found on earth. The chitin utilization locus in Flavobacterium johnsoniae, which encodes necessary proteins for complete enzymatic depolymerization of crystalline chitin, has rec
Externí odkaz:
https://doaj.org/article/68af64a224324c0884485b7326924765
Autor:
Bjarni Ásgeirsson, Sigurbjörn Markússon, Sigríður S. Hlynsdóttir, Ronny Helland, Jens G. Hjörleifsson
Publikováno v:
Biochemistry and Biophysics Reports, Vol 24, Iss , Pp 100830- (2020)
Background: Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Methods: Cyclohexylamine inhibited four di
Externí odkaz:
https://doaj.org/article/76850bdaa0ea4fb387b62e19553df988
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Abstract The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal structure of MZ0003, which reveals that residues co
Externí odkaz:
https://doaj.org/article/866378946e154b7289823b30b9e57bf9
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0217713 (2019)
N-acetylneuraminate lyases (NALs) are enzymes that catalyze the reversible cleavage and synthesis of sialic acids. They are therefore commonly used for the production of these high-value sugars. This study presents the recombinant production, togethe
Externí odkaz:
https://doaj.org/article/bb69f2905eda4cc186afe82c42a86934
Autor:
Kenneth A Johnson, Thomas Ve, Oivind Larsen, Rolf B Pedersen, Johan R Lillehaug, Harald B Jensen, Ronny Helland, Odd A Karlsen
Publikováno v:
PLoS ONE, Vol 9, Iss 2, p e87750 (2014)
CorA is a copper repressible protein previously identified in the methanotrophic bacterium Methylomicrobium album BG8. In this work, we demonstrate that CorA is located on the cell surface and binds one copper ion per protein molecule, which, based o
Externí odkaz:
https://doaj.org/article/6f852697dc74462c812ad7964fb6f004
The Methylococcus capsulatus (Bath) secreted protein, MopE*, binds both reduced and oxidized copper.
Autor:
Thomas Ve, Karina Mathisen, Ronny Helland, Odd A Karlsen, Anne Fjellbirkeland, Åsmund K Røhr, K Kristoffer Andersson, Rolf-Birger Pedersen, Johan R Lillehaug, Harald B Jensen
Publikováno v:
PLoS ONE, Vol 7, Iss 8, p e43146 (2012)
Under copper limiting growth conditions the methanotrophic bacterium Methylococcus capsulatus (Bath) secrets essentially only one protein, MopE*, to the medium. MopE* is a copper-binding protein whose structure has been determined by X-ray crystallog
Externí odkaz:
https://doaj.org/article/f1eb7d75ef9146b8a6e2a5ea03fda97c
Publikováno v:
FEBS Open Bio
This study highlights the importance of a hydrogen bonding network between the subunits in a homodimeric enzyme involving a large loop for maintaining a high catalytic rate. Although increased local flexibility in the opposite subunit increased when
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fb5fc69f333ef61e8fbad57fdaa4a25
https://hdl.handle.net/10037/20502
https://hdl.handle.net/10037/20502
The role of surface loops in mediating communication through residue networks is still a relatively poorly understood part of cold-adaptation of enzymes, especially in terms of their quaternary interactions. Alkaline phosphatase (AP) from the psychro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5c28f55c2ee57d05f9875a7d5920fbe
https://doi.org/10.1101/2020.10.27.357921
https://doi.org/10.1101/2020.10.27.357921
Autor:
Bjarni, Ásgeirsson, Sigurbjörn, Markússon, Sigríður S, Hlynsdóttir, Ronny, Helland, Jens G, Hjörleifsson
Publikováno v:
Biochemistry and Biophysics Reports
Background Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Methods Cyclohexylamine inhibited four diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e72db72df0182263a9bd1919efc2f844
https://pubmed.ncbi.nlm.nih.gov/33102813
https://pubmed.ncbi.nlm.nih.gov/33102813
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
'Scientific Reports ', vol: 7, pages: 17278-1-17278-10 (2017)
Scientific Reports
'Scientific Reports ', vol: 7, pages: 17278-1-17278-10 (2017)
Scientific Reports
Source at http://dx.doi.org/10.1038/s41598-017-17677-4 . The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f58a4c0b2da9106d3fca8659a615d5a
https://doaj.org/article/866378946e154b7289823b30b9e57bf9
https://doaj.org/article/866378946e154b7289823b30b9e57bf9