Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Ronan D. O'Kennedy"'
Publikováno v:
Biotechnology progressREFERENCES. 35(6)
In this study, we have demonstrated that the type and feeding regimen of amino acids have a significant impact on the quality as well as the quantity of DNA vectors produced. Nutrient pool and factorial design experiments were carried out in order to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91db009317d72024b8b42ddd602485fe
https://pubmed.ncbi.nlm.nih.gov/31298810
https://pubmed.ncbi.nlm.nih.gov/31298810
Autor:
Andrew F. Day, Simyee Kong, Nigel J. Titchener-Hooker, Parviz Ayazi Shamlou, Ronan D. O'Kennedy
Publikováno v:
Journal of Chemical Technology & Biotechnology. 84:696-701
BACKGROUND: Viscosity – time plots for plasmid-bearing E. coli cells undergoing alkaline lysis are reported in this study. The plots demonstrate generic features that reflect the progress of fermentation and allow an assessment of the genomic DNA d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::de0843c253a99ccd672f9045d8c1515f
https://doi.org/10.1002/jctb.2101
https://doi.org/10.1002/jctb.2101
Publikováno v:
Chemical Engineering Science. 61:4860-4870
Automated experimentation in microwell plate formats is widely used in high throughput drug discovery. Such approaches are now being considered for the study of bioprocess unit operations in order to speed the delivery of new medicines to market. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1760b5c31765f0846bcead16b292a51c
https://doi.org/10.1016/j.ces.2006.02.001
https://doi.org/10.1016/j.ces.2006.02.001
Autor:
Anthony J. Nealon, Karen E. Willson, Tim M. Clayton, Nigel J. Titchener-Hooker, Ronan D. O'Kennedy, Samuel C. R. Pickering, Gary J. Lye
Publikováno v:
Biotechnology Progress. 21:283-291
This study examines the utility of an automated liquid handling robot integrated with a microwell plate reader to enable the rapid acquisition of bioprocess kinetic data. The relationship between the key parameters for liquid handling accuracy and pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b040ede480dee02e4f7ee62e886657f3
https://doi.org/10.1021/bp049868+
https://doi.org/10.1021/bp049868+
Publikováno v:
Trends in Biotechnology. 18:296-305
Plasmid-based genes offer promise for a new generation of vaccines and for gene therapy, but the size and character of plasmids pose new challenges to biochemical engineers. By acknowledging these and using bioprocess-design information based on fund
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ddbe388b76cfb19bde5f80005f0b8e4
https://doi.org/10.1016/s0167-7799(00)01446-3
https://doi.org/10.1016/s0167-7799(00)01446-3
Autor:
M. Tservistas, P. York, M.S. Levy, M.Y.A. Lo-Yim, Mike Hoare, G.O. Humphrey, Ronan D. O'Kennedy
Publikováno v:
Biotechnology & Bioengineering. 72:12-18
The invention of novel drugs based on biological macromolecules requires the development of specialized formulation methods. Supercritical fluid technology offers the possibility to produce dry powder formulations suitable for inhalation or needle-fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1fc981fe9dce117a792a86080b54b56e
https://doi.org/10.1002/1097-0290(20010105)72:1<12::aid-bit2>3.0.co
https://doi.org/10.1002/1097-0290(20010105)72:1<12::aid-bit2>3.0.co
Autor:
John W. Patching, Ronan D. O'Kennedy
Publikováno v:
Journal of Biotechnology. 69:203-214
A recombinant strain of Saccharomyces cerevisiae containing a plasmid-encoded lacZ gene from Escherichia coli was grown for 420 generations under selective conditions in glucose-limited continuous culture. A ura3-based auxotrophic system was used to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cebd4b6e2a003ffddcc74c306a33ebec
https://doi.org/10.1016/s0168-1656(99)00047-4
https://doi.org/10.1016/s0168-1656(99)00047-4
Publikováno v:
Biotechnology and Bioengineering. 66:195-201
A sensitive fluorescence-based method for monitoring plasmid DNA during production was investigated. This simple method of assaying for plasmid DNA allows rapid monitoring of plasmid yields from a recombinant Escherichia coli fed-batch fermentation.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e03083c09f8595187c9ed3798162360
https://doi.org/10.1002/(sici)1097-0290(1999)66:3<195::aid-bit8>3.0.co
https://doi.org/10.1002/(sici)1097-0290(1999)66:3<195::aid-bit8>3.0.co
Publikováno v:
Process Biochemistry. 33:555-569
The effect of pH on growth rates and sulphide toxicity thresholds of a variety of key bacterial groups involved in anaerobic digestion is described. At pH 7·0–7·5, the growth rates of methane-producing bacteria (MPB) and sulphate-reducing bacteri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0506048ac166b81ce70992b0c0771449
https://doi.org/10.1016/s0032-9592(98)00018-1
https://doi.org/10.1016/s0032-9592(98)00018-1
Autor:
John W. Patching, Ronan D. O'Kennedy
Publikováno v:
Biotechnology Letters. 18:713-718
Transformation-induced slow growth phenotype (SGP) in yeast is repressed in the presence of 2μm plasmids. A full 2μm-sequence-based recombinant plasmid (pJB502) was found to be more stable in a 2μm-free- [cir∘] strain of Saccharomyces cerevisiae
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bb9a4868f07dd23c17c26b059e329f7
https://doi.org/10.1007/bf00130771
https://doi.org/10.1007/bf00130771