Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Autor: Ana Alice Maia Gonçalves, Anna Julia Ribeiro, Carlos Ananias Aparecido Resende, Carolina Alves Petit Couto, Isadora Braga Gandra, Isabelle Caroline dos Santos Barcelos, Jonatas Oliveira da Silva, Juliana Martins Machado, Kamila Alves Silva, Líria Souza Silva, Michelli dos Santos, Lucas da Silva Lopes, Mariana Teixeira de Faria, Sabrina Paula Pereira, Sandra Rodrigues Xavier, Matheus Motta Aragão, Mayron Antonio Candida-Puma, Izadora Cristina Moreira de Oliveira, Amanda Araujo Souza, Lais Moreira Nogueira, Mariana Campos da Paz, Eduardo Antônio Ferraz Coelho, Rodolfo Cordeiro Giunchetti, Sonia Maria de Freitas, Miguel Angel Chávez-Fumagalli, Ronaldo Alves Pinto Nagem, Alexsandro Sobreira Galdino

Publikováno v: Microbial Cell Factories, Vol 23, Iss 1, Pp 1-32 (2024)

Abstract Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnos

Externí odkaz: https://doaj.org/article/04128f35e0ba45f6b080b530e11fda58

Proteus: An algorithm for proposing stabilizing mutation pairs based on interactions observed in known protein 3D structures

Autor: José Renato M. S. Barroso, Diego Mariano, Sandro R. Dias, Rafael E. O. Rocha, Lucianna H. Santos, Ronaldo A. P. Nagem, Raquel C. de Melo-Minardi

Publikováno v: BMC Bioinformatics, Vol 21, Iss 1, Pp 1-21 (2020)

Abstract Background Protein engineering has many applications for industry, such as the development of new drugs, vaccines, treatment therapies, food, and biofuel production. A common way to engineer a protein is to perform mutations in functionally

Externí odkaz: https://doaj.org/article/b4f5f3b3c22b45a991ff5116c8fee2b1

The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions.

Autor: Danielle Gomes Passos Silva, Selma da Silva Santos, Sheila C Nardelli, Isabela Cecília Mendes, Anna Cláudia Guimarães Freire, Bruno Marçal Repolês, Bruno Carvalho Resende, Héllida Marina Costa-Silva, Verônica Santana da Silva, Karla Andrade de Oliveira, Camila Franco Batista Oliveira, Liza Figueiredo Felicori Vilela, Ronaldo Alves Pinto Nagem, Glória Regina Franco, Andrea Mara Macedo, Sergio Danilo Junho Pena, Erich Birelli Tahara, Policarpo Ademar Sales Junior, Douglas Souza Moreira, Santuza Maria Ribeiro Teixeira, Richard McCulloch, Stela Virgilio, Luiz Ricardo Orsini Tosi, Sergio Schenkman, Luciana Oliveira Andrade, Silvane Maria Fonseca Murta, Carlos Renato Machado

Publikováno v: PLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006875 (2018)

In Trypanosoma cruzi, the etiologic agent of Chagas disease, Rad51 (TcRad51) is a central enzyme for homologous recombination. Here we describe the different roles of TcRad51 in DNA repair. Epimastigotes of T. cruzi overexpressing TcRAD51 presented a

Externí odkaz: https://doaj.org/article/36c29e39f2654429899460add329f872

Correction: Antigenicity and Protective Efficacy of a Leishmania Amastigote-specific Protein, Member of the Super-oxygenase Family, against Visceral Leishmaniasis.

Autor: Vivian T Martins, Miguel A Chávez-Fumagalli, Lourena E Costa, Adriana M C Canavaci, Paula S Lage, Daniela P Lage, Mariana C Duarte, Diogo G Valadares, Rubens D M Magalhães, Tatiana G Ribeiro, Ronaldo A P Nagem, Wanderson D Darocha, Wiliam C B Régis, Manuel Soto, Eduardo A F Coelho, Ana Paula Fernandes, Carlos A P Tavares

Publikováno v: PLoS Neglected Tropical Diseases, Vol 7, Iss 4 (2013)

[This corrects the article on p. e2148 in vol. 7.].

Externí odkaz: https://doaj.org/article/d7ad906be92f4af5912847fd6c0a1358

Antigenicity and Protective Efficacy of a Leishmania Amastigote-specific Protein, Member of the Super-oxygenase Family, against Visceral Leishmaniasis

Autor: Vivian T, Martins, Miguel A, Chávez-Fumagalli, Lourena E, Costa, Adriana M C, Canavaci, Adriana M C C, Martins, Paula S, Lage, Daniela P, Lage, Mariana C, Duarte, Diogo G, Valadares, Rubens D M, Magalhães, Tatiana G, Ribeiro, Ronaldo A P, Nagem, Wanderson D, Darocha, Wiliam C B, Régis, Manuel, Soto, Eduardo A F, Coelho, Ana Paula, Fernandes, Carlos A P, Tavares

Publikováno v: PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2148 (2013)
Digital.CSIC. Repositorio Institucional del CSIC
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Background:The present study aimed to evaluate a hypothetical Leishmania amastigote-specific protein (LiHyp1), previously identified by an immunoproteomic approach performed in Leishmania infantum, which showed homology to the super-oxygenase gene fa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::261c9f5230c17e8285d3c523438dfcff
https://pubmed.ncbi.nlm.nih.gov/23638189