Výsledky vyhledávání - "Ronald W. A. Oliver"

Receptor recognition sites reside in both lobes of human serum transferrin

Autor: Anne B. Mason, John F. Brandts, Ross T. A. MacGillivray, Beatrice M. Tam, Janet A. Lineback, Robert C. Woodworth, Lung-Nan Lin, Brian N. Green, Ronald W. A. Oliver, Kerry J. Savage

Publikováno v: Biochemical Journal. 326:77-85

The binding of iron by transferrin leads to a significant conformational change in each lobe of the protein. Numerous studies have shown that the transferrin receptor discriminates between iron-saturated and iron-free transferrin and that it modulate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57f6e2815bfcb8b060d0dbd879f3aa79
https://doi.org/10.1042/bj3260077

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Production and Isolation of the Recombinant N-Lobe of Human Serum Transferrin from the Methylotrophic YeastPichia pastoris

Autor: Beatrice M. Tam, Brian N. Green, Anne B. Mason, Ross T. A. MacGillivray, Robert C. Woodworth, Ronald W. A. Oliver, John F. Brandts, Lung-Nan Lin, Alexisann Maxwell

Publikováno v: Protein Expression and Purification. 8:119-125

The N-lobe of human serum transferrin has been expressed in the methylotrophic yeast Pichia pastoris by placing the hTF/2N cDNA under the control of the methanol-inducible alcohol oxidase promoter. Following induction with methanol, the N-lobe was ef

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::337a8a87af1e2e5adc6845cac99f7b95
https://doi.org/10.1006/prep.1996.0081

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Posttranslational processing of recombinant human interferon-γ in animal expression systems

Autor: Brian N. Green, Merlin H. Goldman, Ronald W. A. Oliver, Robert B. Freedman, Nigel Jenkins, David C. James, Mike Hoare

Publikováno v: Protein Science. 5:331-340

We have characterized the heterogeneity of recombinant human interferon-gamma (IFN-gamma) produced by three expression systems: Chinese hamster ovary cells, the mammary gland of transgenic mice, and baculovirus-infected Spodoptera frugiperda (Sf9) in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::436b8411417c8526de2330b8d57b2d7c
https://doi.org/10.1002/pro.5560050217

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Mass Spectrometic Composition, Molecular Mass and Oxygen Binding ofMacrobdella decoraHemoglobin and its Tetramer and Monomer Subunits

Autor: Emory H. Braswell, Ronald W. A. Oliver, Askar R. Kuchumov, Brian N. Green, Serge N. Vinogradov, H. Malte, Roy E. Weber, Pawan K. Sharma

Publikováno v: Journal of Molecular Biology. 251:703-720

The hexagonal bilayer hemoglobin (Hb) of the leech Macrobdella decora has a equilibrium sedimentation mass of 3544(±80) kDa. Maximum entropy analysis of the electrospray ionization mass spectra of the Hb show three groups of peaks: two peaks of equa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2348b494737bae4d971259a4f5bea41
https://doi.org/10.1006/jmbi.1995.0466

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Mechanism of production of ions in electrospray mass spectrometry

Autor: Ronald W. A. Oliver, D.S. Ashton, C. R. Beddell, D. J. Cooper, B. N. Green

Publikováno v: Organic Mass Spectrometry. 28:721-728

A mechanism for the production of multiply charged molecular ion species in electrospray mass spectrometry (ES-MS) is still required. A concise discussion of a recently published ionic solution equilibrium model offering a partial mechanism is presen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d13b36c3f5c400163f3212deb8068e1f
https://doi.org/10.1002/oms.1210280612

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Expression of glycosylated and nonglycosylated human transferrin in mammalian cells. Characterization of the recombinant proteins with comparison to three commercially available transferrins

Autor: Walter D. Funk, Michael K. Miller, Kerry J. Savage, Anne B. Mason, David K. Banfield, Ronald W. A. Oliver, Ross T. A. MacGillivray, Robert C. Woodworth, Brian N. Green

Publikováno v: Biochemistry. 32:5472-5479

The coding sequence for human serum transferrin was assembled from restriction fragments derived from a full-length cDNA clone isolated from a human liver cDNA library. The assembled clone was inserted into the expression vector pNUT and stably trans

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bfadc2dc715ba4eb4ddc0b0cf26c39a
https://doi.org/10.1021/bi00071a025

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Some electrospray mass spectrometric evidence for the existence of covalentO‐acyl enzyme intermediates

Autor: Kevin J. Welham, Brian N. Green, Ronald W. A. Oliver, Christopher R. Beddell, David J. Cooper, D.S. Ashton

Publikováno v: FEBS Letters. 292:201-204

Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (α-chymotrypsin, subtilisin Carlsberg and subtilisin BPN′) before, during and after completion of the hydrolytic react

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3aa895f6045494b22c18ee1aae5cbc42
https://doi.org/10.1016/0014-5793(91)80867-3

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