Expression and Cleavage of Middle East Respiratory Syndrome Coronavirus nsp3-4 Polyprotein Induce the Formation of Double-Membrane Vesicles That Mimic Those Associated with Coronaviral RNA Replication

Autor: Diede Oudshoorn, Kevin Rijs, Ronald W. A. L. Limpens, Kevin Groen, Abraham J. Koster, Eric J. Snijder, Marjolein Kikkert, Montserrat Bárcena

Publikováno v: mBio, Vol 8, Iss 6 (2017)

ABSTRACT Betacoronaviruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV), are important pathogens causing potentially lethal infections in humans and animals. Coronavirus RNA synthesis is thought to be associated with replication o

Externí odkaz: https://doaj.org/article/a3a7cf2cc99549698cbfbe0f6767b044

The Transformation of Enterovirus Replication Structures: a Three-Dimensional Study of Single- and Double-Membrane Compartments

Autor: Ronald W. A. L. Limpens, Hilde M. van der Schaar, Darshan Kumar, Abraham J. Koster, Eric J. Snijder, Frank J. M. van Kuppeveld, Montserrat Bárcena

Publikováno v: mBio, Vol 2, Iss 5 (2011)

ABSTRACT All positive-strand RNA viruses induce membrane structures in their host cells which are thought to serve as suitable microenvironments for viral RNA synthesis. The structures induced by enteroviruses, which are members of the family Picorna

Externí odkaz: https://doaj.org/article/9d65c6db481e4110a79dc3ac16cd8fbf

Organoid-based expansion of patient-derived primary alveolar type 2 cells for establishment of alveolus epithelial Lung-Chip cultures

Autor: Sander van Riet, Annemarie van Schadewijk, P. Padmini S. J. Khedoe, Ronald W. A. L. Limpens, Montserrat Bárcena, Jan Stolk, Pieter S. Hiemstra, Anne M. van der Does

Publikováno v: American Journal of Physiology-Lung Cellular and Molecular Physiology, 322(4), L526-L538. AMER PHYSIOLOGICAL SOC

Development of effective treatment strategies for lung tissue destruction as seen in emphysema would greatly benefit from representative human in vitro models of the alveolar compartment. Studying how cellular cross talk and/or (altered) biomechanica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::659a7546b671b76a19e73821a5f93dcc
https://hdl.handle.net/1887/3514356

Estradiol-driven metabolism in transwomen associates with reduced circulating extracellular vesicle microRNA-224/452

Autor: Hetty C M Sips, Huayu Zhang, Patrick C.N. Rensen, Rienk Nieuwland, Ton J. Rabelink, Wendy Stam, Anton Jan van Zonneveld, Sander Kooijman, Maartje Klaver, Ronald W. A. L. Limpens, Barend W. Florijn, Martin den Heijer, Jurriën Prins, Maaike Hanegraaf, Roel Bijkerk, Jacques M.G.J. Duijs, Bas B. van Rijn, Eline N. Kuipers

Publikováno v: European Journal of Endocrinology, 185(4), 539-552. BioScientifica Ltd.
European Journal of Endocrinology, 185(4), 539-552. Bioscientifica Ltd
European Journal of Endocrinology
Florijn, B W, Duijs, J M G J, Klaver, M, Kuipers, E N, Kooijman, S, Prins, J, Zhang, H, Sips, H C M, Stam, W, Hanegraaf, M, Limpens, R W A L, Nieuwland, R, van Rijn, B B, Rabelink, T J, Rensen, P C N, den Heijer, M, Bijkerk, R & van Zonneveld, A J 2021, ' Estradiol-driven metabolism in transwomen associates with reduced circulating extracellular vesicle microRNA-224/452 ', European Journal of Endocrinology, vol. 185, no. 4, pp. 539-552 . https://doi.org/10.1530/EJE-21-0267
European Journal of Endocrinology, 185(4), 539-552. BIOSCIENTIFICA LTD

Objective Sex steroid hormones like estrogens have a key role in the regulation of energy homeostasis and metabolism. In transwomen, gender-affirming hormone therapy like estradiol (in combination with antiandrogenic compounds) could affect metabolis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adc0ba02da264f0cd6a21c389ba67297
https://research.vumc.nl/en/publications/24631769-627e-4df1-9dbc-1d5f8df68840

A Bacterially-Expressed Recombinant Envelope Protein from Usutu Virus Induces Neutralizing Antibodies in Rabbits

Autor: Montserrat Bárcena, Ernst J. Verschoor, Amy van Dijke, Babs E. Verstrepen, Zahra Fagrouch, Willy M. J. M. Bogers, Gesine Cornellissen, Janet Hirsch, Soesjiel Dwarka, Ronald W. A. L. Limpens, Boyd Aaldijk, Bart W. Faber, Kinga P. Böszörményi, Gwendoline Kiemenyi Kayere, Roberto Rodriguez Garcia, Bram Koster, Nicole Heijmans, Clemens H. M. Kocken

Publikováno v: Vaccines
Volume 9
Issue 2
Vaccines, 9(2). MDPI
Vaccines, Vol 9, Iss 157, p 157 (2021)

Background: Recently, an emerging flavivirus, Usutu virus (USUV), has caused an epidemic among birds in Europe, resulting in a massive die-off in Eurasian blackbirds. Currently found only in Europe and Africa, it can be envisioned that Usutu virus wi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bbd6cc068ce0cc8406df0aabe643bd1
https://pubmed.ncbi.nlm.nih.gov/33669414