Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ronald N. Keener"'
Quantification of protein posttranslational modifications using stable isotope and mass spectrometry
Autor:
Shun Luo, Quanzhou Luo, Xinzhao Grace Jiang, Izydor Apostol, Ronald N. Keener, Xin Zhang, Matthew Jerums, Gang Huang, Jette Wypych, Jeffrey Lewis
Publikováno v:
Analytical Biochemistry. 421:506-516
With the increased attention to quality by design (QbD) for biopharmaceutical products, there is a demand for accurate and precise quantification methods to monitor critical quality attributes (CQAs). To address this need we have developed a mass spe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::104ff1b02d366926db337af5c85a5d7e
https://doi.org/10.1016/j.ab.2011.12.004
https://doi.org/10.1016/j.ab.2011.12.004
Publikováno v:
Journal of Chromatography A. 1190:127-140
This paper details the advancements made in the modeling of open column and packed bed pressure-flow. The theoretical description is a one-dimensional elasticity model. By accounting for the loss of intra-particle porosity through empiricism, and by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7401410f387388263de7ef9c50f26999
https://doi.org/10.1016/j.chroma.2008.02.113
https://doi.org/10.1016/j.chroma.2008.02.113
Publikováno v:
Biotechnology Progress. 20:1146-1158
The packing of compressible biochromatographic resins at large scale suffers from a poor understanding of how column packing method, resin properties, and column geometry impact column performance. To improve understanding, we develop and evaluate a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4f93cb8d35c683414366d08ef6e0e806
https://doi.org/10.1021/bp034279+
https://doi.org/10.1021/bp034279+
Publikováno v:
Biotechnology Progress. 20:1159-1168
We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ed7a29694fd29710067f5ea81b91f47b
https://doi.org/10.1021/bp0342809
https://doi.org/10.1021/bp0342809
Publikováno v:
Biotechnology Progress. 18:587-596
A one-dimensional model of mechanical deformation of compressible chromatography columns is presented. The model is based on linear elasticity and continuum mechanics and is compared to a more complete two-dimensional model and one-dimensional porosi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bee75adbb322fc7cc6ee9edb1c8554a9
https://doi.org/10.1021/bp020051v
https://doi.org/10.1021/bp020051v
Autor:
Ronald N. Keener, Paul J. Kocin, Steven M. Zubrick, David R. Rodenhuis, C. W. King, Andrew Crook, Ellis M. Stanley, Jeremy Hales, Fred Sharrocks, Robert Bornstein, Michael A. Rossetti, J. Christopher Doran, Witold F. Krajewski, Walter F. Dabberdt, Cynthia K. Mueller
Publikováno v:
Bulletin of the American Meteorological Society. 81:2047-2064
The 10th Prospectus Development Team (PDT-10) of the U.S. Weather Research Program was charged with iden- tifying research needs and opportunities related to the short-term prediction of weather and air quality in urban forecast zones. Weather has sp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::894e22e8913d87b86ba7baa311943d9d
https://doi.org/10.1175/1520-0477(2000)081<2047:fiituz>2.3.co
https://doi.org/10.1175/1520-0477(2000)081<2047:fiituz>2.3.co
Autor:
Ronald N. Keener, Xinzhao Grace Jiang, Jessica Gastwirt, Matthew Jerums, Shun Luo, Jette Wypych, Izydor Apostol, Gang Huang, Nimesh Savjani, Jeffrey Lewis, Xin Zhang, Quanzhou Luo
Publikováno v:
Analytical biochemistry. 433(2)
Stable isotope labeling combined with mass spectrometry has been widely used in a diverse set of applications in the biochemistry and biomedical fields. When stable isotope-labeled proteins are produced via metabolic labeling of cell culture, a compr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fd1a54a73b12ef0c456dc0ba0bd4bd2
https://pubmed.ncbi.nlm.nih.gov/23085118
https://pubmed.ncbi.nlm.nih.gov/23085118
Autor:
Izydor Apostol, Matthew Jerums, Xin Zhang, Quanzhou Luo, Ronald N. Keener, Xinzhao Grace Jiang, Jeffrey Lewis, Jette Wypych, Gang Huang, Shun Luo
Publikováno v:
Analytical biochemistry. 421(2)
In this report, we examine the performance of a mass spectrometry (MS)-based method for quantification of protein posttranslational modifications (PTMs) using stable isotope labeled internal standards. Uniform labeling of proteins and highly similar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aaa255a11b2c5a61cbc6ef20a9b7430
https://pubmed.ncbi.nlm.nih.gov/22227056
https://pubmed.ncbi.nlm.nih.gov/22227056
Publikováno v:
Biotechnology progress. 20(4)
The packing of compressible biochromatographic resins at large scale suffers from a poor understanding of how column packing method, resin properties, and column geometry impact column performance. To improve understanding, we develop and evaluate a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2b50955d246729470aea4063bcab0034
https://pubmed.ncbi.nlm.nih.gov/15296442
https://pubmed.ncbi.nlm.nih.gov/15296442
Publikováno v:
Biotechnology progress. 20(4)
We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2efca7ef0fb225e22c13403ec5a9a85d
https://pubmed.ncbi.nlm.nih.gov/15296443
https://pubmed.ncbi.nlm.nih.gov/15296443