Zobrazeno 1 - 10
of 155
pro vyhledávání: '"Ronald J. Doyle"'
Publikováno v:
Journal of Microbiological Methods. 52:285-296
A rapid direct sputum (Sp.) and/or antibody assay, based on immunoblotting and enzyme immunoassay is described. The test can detect mycobacterial antigens or antibodies in clinical specimens from pulmonary tuberculosis (TB) patients. In this study, 8
Publikováno v:
FEMS Immunology & Medical Microbiology. 28:343-346
Coaggregation between pairs of microorganisms was found to be inhibited by chelating agents, such as acetylacetone, citrate, EDTA and carboxymethylcellulose. Assays were conducted on eight pairs of periodontopathogens and one pair consisting of Esche
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1474:346-352
Glucan-binding lectin (GBL) activity of Streptococcus sobrinus was significantly reduced by fluoride in the growth medium. Approximately 1.5 mM fluoride was required for a 50% reduction in GBL activity. In addition to the GBL, several other glucan-bi
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1428:415-423
Addition of fluoride to the growth medium of Streptococcus sobrinus resulted in a loss of glucan-binding lectin activity. Upon removal of fluoride, the bacteria regained their ability to bind glucan in about one generation. Chloramphenicol prevented
Autor:
Itzhak Ofek, Ronald J. Doyle
Attachment to host cells or tissues is often the first step in the establishment of bacterial infections. A complex array of recognition, attachment, and virulence factors is involved in this process, which recent research has greatly illuminated. Th
Publikováno v:
Journal of Biological Chemistry. 272:17880-17886
Type 1 fimbriae are the most common adhesive organelles of Escherichia coli. Because of their virtual ubiquity, previous epidemiological studies have not found a correlation between the presence of type 1 fimbriae and urinary tract infections (UTIs).
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 8:295-302
Several members of the genus Streptococcus, including S. sobrinus, S. cricetus, S. sanguis and S. mutans, are known to produce proteins capable of complexing with α-1,6 glucans. Of all the oral streptococcal groups, S. sobrinus and S. cricetus are t
Publikováno v:
Journal of Microbiological Methods. 25:19-22
Lipoteichoic acids (LTAs) have the ability to form stable complexes with polysaccharides in ethanol solutions. When crude preparations of LTA from Bacillus subtilis and Streptococcus sobrinus were poured over a column of Sephacryl S-200 equilibrated
Autor:
Andreia Popescu, Ronald J. Doyle
Publikováno v:
Biotechnic & Histochemistry. 71:145-151
The Gram stain, the most important stain in microbiology, was described more than a century ago. Only within the past decade, however, has an understanding of its mechanism emerged. It now seems clear that the cell wall of Gram-positive microorganism
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 5:127-134
Solutions of ammonium sulfate (AMS) have been found to aggregate bacteria in a quantifiable and reproducible manner. The rates of aggregation promoted by the AMS of several bacteria obeyed pseudo-first-order kinetics. There was a high correlation bet