Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ronald I. W. Osmond"'
Autor:
Ronald I. W. Osmond, Peter Banks, Michael Francis Crouch, Wendy Goodrich, Antony James Sheehan
Publikováno v:
ASSAY and Drug Development Technologies. 11:108-116
We describe a cellular assay for detection of phosphorylation of endogenous proteins, whereby cells are seeded, treated, and assayed for modulation of phosphorylation in a single microplate well. The procedure is coupled to a rapid, one-wash sandwich
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7807b5164af7a6cfe92580c7cb3b78cc
https://doi.org/10.1089/adt.2012.471
https://doi.org/10.1089/adt.2012.471
Autor:
M. Henry Martin-Harris, Ronald I. W. Osmond, Janet Park, Michael Francis Crouch, Eric Morreale, Vincent J. Dupriez
Publikováno v:
SLAS Discovery. 17:361-369
To expedite G-protein-coupled receptor (GPCR) drug screening studies, cell lines amenable to transfection (e.g. CHO cells) have been widely used as cellular models. These cells can be frozen in a ready-to-use format, allowing screening of a single ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fab2cfe8215ed49f07131b06230ea614
https://doi.org/10.1177/1087057111425859
https://doi.org/10.1177/1087057111425859
Direct detection of the binding of avidin and lactoferrin fluorescent probes to heparinized surfaces
Publikováno v:
Analytical Biochemistry. 339:206-215
We describe the use of two heparin-binding proteins, avidin and lactoferrin, as probes for monitoring the amount of heparin immobilized to plastic surfaces. The proteins were derivatized with either fluorescent labels or europium chelates, enabling s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4509205cb321f4c0d7a5f374ba8854be
https://doi.org/10.1016/j.ab.2005.01.054
https://doi.org/10.1016/j.ab.2005.01.054
Autor:
Lily Moe, Spencer E Skett, Deirdre R. Coombe, Warren C. Kett, Ronald I. W. Osmond, Beverley F. Kinnear
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1620:225-234
The specificity, affinity and stoichiometry of the interaction between avidin and glycosaminoglycans (GAGs) have been investigated using heparin-coated microtiter-plate assays, a filter binding assay and surface plasmon resonance (SPR) analysis using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06bac55a76b1d792612b3a3d5b4762e5
https://doi.org/10.1016/s0304-4165(02)00539-1
https://doi.org/10.1016/s0304-4165(02)00539-1
Publikováno v:
Current opinion in molecular therapeutics. 12(3)
GPCRs are a large class of cell-surface receptors that are involved in a diverse array of biological processes, including many that are critical to diseases. As a result, GPCRs are a major focus for drug discovery research, and have been highly amena
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e7fd7278fb0450a307e1a1a590ee8352
https://pubmed.ncbi.nlm.nih.gov/20521219
https://pubmed.ncbi.nlm.nih.gov/20521219
Autor:
Beverley F. Kinnear, Deirdre R. Coombe, Sandra M. Stevenson, Ricardo L. Mancera, Warren C. Kett, Neha S. Gandhi, Ronald I. W. Osmond
Publikováno v:
Biochemistry. 47(17)
Platelet endothelial cell adhesion molecule 1 (PECAM-1) (CD31), a member of the immunoglobulin (Ig) superfamily of cell adhesion molecules with six Ig-like domains, has a range of functions, notably its contributions to leukocyte extravasation during
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a8a629d937194d3bf81c9b54822e7f0
https://pubmed.ncbi.nlm.nih.gov/18327914
https://pubmed.ncbi.nlm.nih.gov/18327914
Publikováno v:
XXIst International Carbohydrate Symposium 2002.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::73e5bb9dac58c3876e61c6fa2e034ce9
https://doi.org/10.1100/tsw.2002.526
https://doi.org/10.1100/tsw.2002.526
Publikováno v:
XXIst International Carbohydrate Symposium 2002.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b3288d00d8981b50667daf419fea54f3
https://doi.org/10.1100/tsw.2002.421
https://doi.org/10.1100/tsw.2002.421
Publikováno v:
European Journal of Biochemistry
European Journal of Biochemistry, Wiley, 2001, pp.4190-4199
European Journal of Biochemistry, Wiley, 2001, pp.4190-4199
The specificity and kinetics of the interaction between the pathogenesis-related group of thaumatin-like proteins (PR5) in higher plants and (1,3)-beta-D-glucans have been investigated. Two thaumatin-like proteins with 60% amino-acid sequence identit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff6994e2305d44cf055de36cf3dc3228
https://hal.archives-ouvertes.fr/hal-00307698
https://hal.archives-ouvertes.fr/hal-00307698
Autor:
Ronald I. W. Osmond
Publikováno v:
Journal of Biomolecular Screening; Oct2005, Vol. 10 Issue 7, p730-737, 8p