Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Ronald George Schoner"'
Publikováno v:
Bio/Technology. 3:151-154
High-level expression of bovine growth hormone in Escherichia coli results in the formation of distinct cytoplasmic granules that are visible with the phase-contrast microscope. These granules were examined by transmission and scanning electron micro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e89d8d7e5fd8d54e7fe7324b0269b0ed
https://doi.org/10.1038/nbt0285-151
https://doi.org/10.1038/nbt0285-151
Publisher Summary This chapter describes a new strategy for improving the translational efficiency of hybrid mRNAs. To preserve the sequence context around the ribosome-binding site, the two-cistron expression system has been designed. In the system,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a520a43064a308196b8a355980bfefbf
https://doi.org/10.1016/0076-6879(87)53068-3
https://doi.org/10.1016/0076-6879(87)53068-3
Publikováno v:
Gene. 16(1-3)
Four different strains of Escherichia coli and several other bacteria were examined by Southern analysis for the presence of the insertion element IS5 and IS5-like sequences. Variations in the copy number, degree of homology and restriction pattern o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::504cc0354bc6649e65c89576815fc8a3
https://pubmed.ncbi.nlm.nih.gov/6282702
https://pubmed.ncbi.nlm.nih.gov/6282702
Autor:
Rama M. Belagaje, Hansen M. Hsiung, Ronald George Schoner, Brigitte Elisabeth Schoner, Nancy G. Mayne
The conditions necessary for high-level expression of methionyl bovine growth hormone (Met-bGH) in Escherichia coli were investigated. Plasmids were constructed that contain a thermoinducible runaway replicon and either the E. coli tryptophan or lipo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82bbd50e5cfea8088f957ad6f4791198
https://europepmc.org/articles/PMC391712/
https://europepmc.org/articles/PMC391712/
A synthetic two-cistron expression system was constructed for the high-level expression of eukaryotic genes in Escherichia coli. This system was designed to overcome translational inhibition of mRNAs containing eukaryotic sequences. The first cistron
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acc3b09c871317f8ac5af10a23d4fb73
https://europepmc.org/articles/PMC386959/
https://europepmc.org/articles/PMC386959/