Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Ronald, Schoner"'
Publikováno v:
AMB Express, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal pep
Externí odkaz:
https://doaj.org/article/6ca52ccbe2584bf2b29e4e8461bb8c30
Autor:
Liangliang Sun, Norman J. Dovichi, Ronald Schoner, Thomas Albanetti, Guijie Zhu, James B. McGivney, Travis Linkous, Christopher Larkin
Publikováno v:
Biotechnology and Bioengineering. 113:2140-2148
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were
Autor:
Raghavan Venkat, James Savery, Ronald Schoner, Krista Evans, Bhargavi Rajan, Thomas Albanetti, Christopher Groves, Guillermo Miro-Quesada
Publikováno v:
Biotechnology Progress
Regulatory authorities require that cell lines used in commercial production of recombinant proteins must be derived from a single cell progenitor or clone. The limiting dilution method of cell cloning required multiple rounds of low‐density cell p
Publikováno v:
AMB Express, Vol 7, Iss 1, Pp 1-14 (2017)
Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides pla
Autor:
Travis Linkous, Thomas Albanetti, Norman J. Dovichi, Ronald Schoner, James B. McGivney, Christopher Larkin, Zhenbin Zhang
Publikováno v:
Electrophoresis. 38(3-4)
Four methods were compared for analysis of host-cell protein (HCP) impurities in a recombinant mAb. First, CZE-MS/MS was used to analyze the digest of an HCP sample following extraction of the mAb with proteins A and L affinity columns; 220 protein g
Autor:
Guijie, Zhu, Liangliang, Sun, Thomas, Albanetti, Travis, Linkous, Christopher, Larkin, Ronald, Schoner, James B, McGivney, Norman J, Dovichi
Publikováno v:
Biotechnology and bioengineering. 113(10)
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were