Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Ron Skurray"'
Autor:
Neville Firth, Ron Skurray
Publikováno v:
Moleculargeneral genetics : MGG. 232(1)
The Escherichia coli F plasmid gene, traG, is required for two stages of the conjugation process: pilus biosynthesis and mating aggregate stabilization. The nucleotide sequence of traG has been determined and the topology of its product in the cytopl
Publikováno v:
Nucleic Acids Research. 18:4597-4597
Publikováno v:
Plasmid. 17:233-239
DNA-DNA hybridization studies have demonstrated the physical relatedness of the fertility inhibition gene, finO, among both FinO+ and FinO- F-like conjugative plasmids, viz. ColV2-K94, R100-1,R1drd19,R1,R6-5, UCR123, R386, p307, R453, R773, and pIP16
Autor:
Animesh Ray, Ron Skurray
Publikováno v:
Scopus-Elsevier
The leading region of the F plasmid is, by definition, the first part of the plasmid DNA to be transferred to the recipient cell during conjugation. Restriction fragments of the leading region, when cloned into the plasmid vector pACYC184, extended t
Publikováno v:
Scopus-Elsevier
The cloning of the finO region from the antibiotic-resistance plasmid R6-5 is reported. On the basis of DNA deletion analysis and Tn 5 transposon insertional mutagenesis of finO + chimeric plasmids, finO has been located within the coordinates 94.0
Publikováno v:
Scopus-Elsevier
Transcriptional activity associated with the leading region (53.8–66.7F) in F DNA transfer has been shown by RNA-DNA hybridization studies to occur on the anterior segment extending from 59.4 to 66.7F. Promoter-probe analysis of cloned leading regi
Publikováno v:
Gene. 32:251-254
Analysis of Tn1725 insertions in the Pif+ plasmid pRS2496 showed the maximum limits of the F pif region to be between 43.7 and 47.15 on the 100-kb map of the F plasmid. The effect of these insertions on the expression of pif polypeptides indicated th
Autor:
Ron Skurray, Neil Willetts
Publikováno v:
Annual Review of Genetics. 14:41-76
INTRODUCTION ....... . . 41 GENETICS OF F-LIKE CONJUGATION SYSTEMS 45 Genetic Analysis 0/ F ... . ... . ... . . ..... ....... ... . ..... .. ... ... . . ... . . ... ....... . 45 Cloning 0/ tra DNA Segments ......... . .... . . ..... . ..... . . ... 4
Publikováno v:
Molecular and General Genetics MGG. 197:129-136
We describe the molecular cloning of BglII fragments of the hybrid plasmid pRS5 (pSC101 and EcoRI fragments of F; f7, f5, f3 and f6). The clones isolated were examined for the expression of F-specified replication, incompatibility, mobilization and i
Autor:
Animesh Ray, Ron Skurray
Publikováno v:
Scopus-Elsevier
A segment of the F plasmid DNA, located between the origin of transfer and the primary F replication region, is the first to enter the recipient cell during conjugation.PstI,SalI, andSmaI restriction endonuclease sites have been mapped within this le