Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Ron Gillespie"'
Autor:
Dean Pettit, Kevin J. Whaley, Jane Ennis, Michael T. Trentalange, Richard S. Rogers, Danielle Pace, Alison J. Gillespie, Brittney Livingston, Edith Nalbandian, Chris Crowell, Kelly K. Arthur, Ron Gillespie, Michael H. Pauly, Yan Brodsky, Ken Timmons, James N Thomas, Larry Zeitlin, Smidt Pauline S, Clark Rutilio H
Publikováno v:
mAbs. 8:347-357
From March 2014 through February 2015, the Ebola virus spread rapidly in West Africa, resulting in almost 30,000 infections and approximately 10,000 deaths. With no approved therapeutic options available, an experimental antibody cocktail known as ZM
Autor:
Kiyoshi Fujimori, Nathan E. Lewis, Julia Bach, Ron Gillespie, Rebecca E. Mccoy, Kenneth M. Prentice, Catherine M. Eakin, Lisa Connell-Crowley
Publikováno v:
mAbs. 5:974-981
Process control of protein therapeutic manufacturing is central to ensuring the product is both safe and efficacious for patients. In this work, we investigate the cause of pink color variability in development lots of monoclonal antibody (mAb) and F
Autor:
Dan Leiske, Nathaniel Lewis, Ron Gillespie, Jyoti Velayudhan, Lisa Connell-Crowley, Danielle Pace, Amanda Rohrbach, Tina Wu
Publikováno v:
Biotechnology progress. 32(5)
N-linked Fc glycosylation of IgG1 monoclonal antibody therapeutics can directly influence their mechanism of action by impacting IgG effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity
Publikováno v:
Journal of Chromatography A. 1251:101-110
Cation exchange chromatography of an aglycosylated IgG1 resulted in two distinct peaks during gradient elution. The early eluting peak contained
Autor:
Houman Bashiri, Ron Gillespie, Houman Dehghani, Shivanthi Chinniah, Peter Hinckley, Lisa Connell-Crowley, Thao Nguyen, Julia Bach, Suresh Vunnum, John Moscariello, Ganesh Vedantham
Publikováno v:
Biotechnology and Bioengineering. 109:157-165
One measure taken to ensure safety of biotherapeutics produced in mammalian cells is to demonstrate the clearance of potential viral contaminants by downstream purification processes. This paper provides evidence that cation exchange chromatography (
Publikováno v:
Biotechnology Progress. 27:1054-1067
Crystallization holds the potential to be used for protein purification and low-viscosity drug substance and drug product formulations. Twenty-two different proteins (20 monoclonal antibodies and two Fc-fusions) were examined to determine the breadth
Publikováno v:
Biotechnology and bioengineering. 110(7)
High throughput screening (HTS) of chromatography resins can accelerate downstream process development by rapidly providing information on product and impurity partitioning over a wide range of experimental conditions. In addition to the removal of t
Autor:
Randal R. Ketchem, Jay B. Jones, Theresa Martinez, Amy Guo, Martin J. Allen, Ron Gillespie, Danielle Pace, Yuling Zhang, Alain Balland, Mei Han
Publikováno v:
Biochemistry. 47(28)
In this communication we present the detailed disulfide structure of IgG2 molecules. The consensus structural model of human IgGs represents the hinge region positioned as a flexible linker connecting structurally isolated Fc and Fab domains. IgG2 mo