Zobrazeno 1 - 10
of 311
pro vyhledávání: '"Romas J Kazlauskas"'
Autor:
Per Berglund
Publikováno v:
ChemBioChem. 7:1280-1280
Hydrolases in Organic Synthesis: Regio- and Stereoselective Biotransformation : By Uwe T. Bornscheuer and Romas J. Kazlauskas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2235a468b7288220534b679a0f49973e
https://doi.org/10.1002/cbic.200600269
https://doi.org/10.1002/cbic.200600269
Publikováno v:
International Journal of Biological Macromolecules. 222:2341-2352
Cyclization of proteins using SpyTag/SpyCatcher is a novel approach to increase their thermal stability. In this paper, we test this approach on two β-galactosidases from Bacillus circulans, BgaB and BgaC, and find that BgaB was stabilized while Bga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d067328b257ee1df50d6df228b8940
https://doi.org/10.1016/j.ijbiomac.2022.10.021
https://doi.org/10.1016/j.ijbiomac.2022.10.021
Autor:
Bryan J Jones, Robert L Evans, Nathan J Mylrea, Debayan Chaudhury, Christine Luo, Bo Guan, Colin T Pierce, Wendy R Gordon, Carrie M Wilmot, Romas J Kazlauskas
Publikováno v:
PLoS ONE, Vol 15, Iss 6, p e0235341 (2020)
Hydroxynitrile lyases (HNL's) belonging to the α/β-hydrolase-fold superfamily evolved from esterases approximately 100 million years ago. Reconstruction of an ancestral hydroxynitrile lyase in the α/β-hydrolase fold superfamily yielded a catalyti
Externí odkaz:
https://doaj.org/article/1032b638bceb4ed6a0096543bc797ca2
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0147024 (2016)
Engineering faster cellulose deconstruction is difficult because it is a complex, cooperative, multi-enzyme process. Here we use experimental evolution to select for populations of Trichoderma citrinoviride that deconstruct up to five-fold more cellu
Externí odkaz:
https://doaj.org/article/7f375656d77a4abb9a5d3bb27241d225
Publikováno v:
Biotechnol Bioprocess Eng
Ionic liquids, also called molten salts, are mixtures of cations and anions that melt below 100 °C. Typical ionic liquids are dialkylimidazolium cations with weakly coordinating anions such as [MeOSO(3)] or [PF(6)]. Advanced ionic liquids such as ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed7eee559e445e074113f53d1f72799a
https://pubmed.ncbi.nlm.nih.gov/34290544
https://pubmed.ncbi.nlm.nih.gov/34290544
Publikováno v:
Science Advances
A designed protein complex processively copies one strand of a plasmid with errors to speed up molecular evolution in vivo.
Extensive exploration of a protein’s sequence space for improved or new molecular functions requires in vivo evolution
Extensive exploration of a protein’s sequence space for improved or new molecular functions requires in vivo evolution
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22267d21f3b892367211f1f5c152c988
https://doi.org/10.1126/sciadv.abg8712
https://doi.org/10.1126/sciadv.abg8712
Autor:
Romas J. Kazlauskas, Hiroyuki Imanishi, Yoichi Toyokawa, Shota Isogai, Akira Nishimura, Xiao Yi, Hiroshi Takagi, Jirasin Koonthongkaew, Tomonori Matsushita
Publikováno v:
Applied and Environmental Microbiology
Homocitrate synthase (HCS) catalyzes the aldol condensation of 2-oxoglutarate (2-OG) and acetyl coenzyme A (AcCoA) to form homocitrate, which is the first enzyme of the lysine biosynthetic pathway in the yeast Saccharomyces cerevisiae. The HCS activi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74ba88d389a72d41c23fcaa697270e8f
https://pubmed.ncbi.nlm.nih.gov/33990312
https://pubmed.ncbi.nlm.nih.gov/33990312
Publikováno v:
Methods in enzymology. 643
The consensus sequence approach to predicting stabilizing substitutions in proteins rests on the notion that conserved amino acids are more likely to contribute to the stability of a protein fold than non-conserved amino acids. To implement a predict
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2ebf102e49e095e8493f9cc7bcd6356a
https://pubmed.ncbi.nlm.nih.gov/32896278
https://pubmed.ncbi.nlm.nih.gov/32896278
The consensus sequence approach to predicting stabilizing substitutions in proteins rests on the notion that conserved amino acids are more likely to contribute to the stability of a protein fold than non-conserved amino acids. To implement a predict
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8798d13f6dc8824f1bef16c720878dd9
https://doi.org/10.1101/2020.06.29.178418
https://doi.org/10.1101/2020.06.29.178418
The consensus sequence approach to predicting stabilizing substitutions in proteins rests on the notion that conserved amino acids are more likely to contribute to the stability of a protein fold than non-conserved amino acids. To implement a predict
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aaf0c5485c815af5599a92405038813f
https://doi.org/10.1016/bs.mie.2020.07.010
https://doi.org/10.1016/bs.mie.2020.07.010