Assessment of the Alveolar Capillary Network in the Postnatal Mouse Lung in 3D Using Serial Block-Face Scanning Electron Microscopy

Autor: Tobias Buchacker, Christian Mühlfeld, Christoph Wrede, Willi L. Wagner, Richard Beare, Matt McCormick, Roman Grothausmann

Publikováno v: Frontiers in Physiology, Vol 10 (2019)

The alveolar capillary network (ACN) has a large surface area that provides the basis for an optimized gas exchange in the lung. It needs to adapt to morphological changes during early lung development and alveolarization. Structural alterations of t

Externí odkaz: https://doaj.org/article/c3d28cd33a6f45d6893fc40337d2373c

Evaluating Registrations of Serial Sections With Distortions of the Ground Truths

Autor: Michael Guthe, Simone Gaffling, David Haberthür, Jaan Toelen, Oleg Lobachev, Christoph Wrede, Lars Knudsen, Takuya Funatomi, Roman Grothausmann, Reinhold Förster, Ruslan Hlushchuk, Thomas Salaets, Christian Mühlfeld, Alexander Pfaffenroth

Publikováno v: Lobachev, Oleg; Funatomi, Takuya; Pfaffenroth, Alexander; Forster, Reinhold; Knudsen, Lars; Wrede, Christoph; Guthe, Michael; Haberthür, David; Hlushchuk, Ruslan; Salaets, Thomas; Toelen, Jaan; Gaffling, Simone; Mühlfeld, Christian; Grothausmann, Roman (2021). Evaluating registrations of serial sections with distortions of the ground truths. IEEE Access, 9, pp. 152514-152535. IEEE 10.1109/ACCESS.2021.3124341
IEEE Access, Vol 9, Pp 152514-152535 (2021)

Registration of histological serial sections is a challenging task. Serial sections exhibit distortions and damage from sectioning. Missing information on how the tissue looked before cutting makes a realistic validation of 2D registrations extremely

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae72b912ec320912c7199a8fd4e254f2
http://hdl.handle.net/10061/14906

Combination of mu CT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study

Autor: David Haberthür, Jonas Labode, Christina Brandenberger, Pablo Hernandez-Cerdan, Andre Gie, Roman Grothausmann, Ruslan Hlushchuk, Willi L. Wagner, Thomas Salaets, Christian Mühlfeld, Jaan Toelen, Oleg Lobachev

Publikováno v: Histochemistry and Cell Biology

Various lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a45fbf06231feae20afeaf3b50d2196d
https://lirias.kuleuven.be/handle/123456789/668356

Using electron microscopes to look into the lung

Autor: Roman Grothausmann, Jan Hegermann, Matthias Ochs, Lars Knudsen, Christian Mühlfeld, Christoph Wrede

Publikováno v: Histochemistry and Cell Biology. 146:695-707

In the nineteenth century, there was a dispute about the existence of a lung alveolar epithelium which remained unsolved until the invention of electron microscopy (EM) and its application to the lung. From the early 1960s, Ewald Weibel became the ma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6717413d5bd10d70dd5bb27493221e9
https://doi.org/10.1007/s00418-016-1502-z

Recent developments in 3-D reconstruction and stereology to study the pulmonary vasculature

Autor: Lars Knudsen, Tobias Buchacker, Roman Grothausmann, Christoph Wrede, Christian Mühlfeld, Matthias Ochs

Publikováno v: American journal of physiology. Lung cellular and molecular physiology. 315(2)

Alterations of the pulmonary vasculature are an important feature of human lung diseases such as chronic obstructive pulmonary disease, pulmonary hypertension, and bronchopulmonary dysplasia. Experimental studies to investigate the pathogenesis or a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72ecb69499b1cf19f9e7f737c72d7645
https://pubmed.ncbi.nlm.nih.gov/29644892