Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Rolf Furter"'
Autor:
Rolf Furter
Publikováno v:
Protein Science. 7:419-426
Site-directed incorporation of the amino acid analogue p-fluoro-phenylalanine (p-F-Phe) was achieved in Escherichia coli. A yeast suppressor tRNA(Phe)amber/phenylalanyl-tRNA synthetase pair was expressed in an analogue-resistant E. coli strain to dir
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1274:59-66
Michaelis- and dissociation constants of sarcomeric mitochondrial creatine kinase (Mi b -CK) in solution were determined by enzyme assay and compared to those of cytosolic MM-CK under identical conditions at pH 7.4 and 25°C. Saturation transfer 31 P
Publikováno v:
Protein Science. 5:320-330
Creatine kinase (CK) has been postulated to consist of two flexibly hinged domains. A previously demonstrated protease-sensitive site in M-CK (Morris & Jackson, 1991) has directed our attempts to dissect mitochondrial CK (Mi-CK) into two protein frag
Publikováno v:
Protein Science. 3:1058-1068
The 5 tryptophan residues of chicken sarcomeric mitochondrial creatine kinase (Mib-CK) were individually replaced by phenylalanine or cysteine using site-directed mutagenesis. The mutant proteins were analyzed by enzyme kinetics, fluorescence spectro
Publikováno v:
Nucleic Acids Research. 22:4932-4936
The yeast shi mutation affects the spacing between the TATA promoter element and transcription initiation sites; for the H2B and ADH1 genes, a series of start sites located approximately 50-80 bp downstream of TATA is used in addition to the wild-typ
Publikováno v:
Biochemistry. 32:7022-7029
Chemical modification of rabbit muscle creatine kinase (CK) with thiol-specific reagents led to partial or complete inactivation of the enzyme. Using site-directed mutagenesis, we have substituted the corresponding reactive Cys278 in the chicken card
Publikováno v:
Biochemistry. 31:10817-10824
Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require
Autor:
Ziegler Hugo, Isenring Hans Peter, Peter Zobrist, Petr Masner, Christopher C. Steel, Gottfried Oesterhelt, Rolf Furter
Publikováno v:
Pesticide Science. 35:339-347
The activity of five experimental fungicides combining structural elements responsible for sterol C-14 demethylase inhibition and sterol nuclear double-bond transformations has been investigated by examining sterol accumulation in Ustilago maydis (DC
Publikováno v:
Journal of General Microbiology. 137:2797-2810
Enzymological evidence has been sought for the purported involvement of chitinolysis in vegetative growth of filamentous fungi. A procedure has been developed for the production of fast growing and morphologically homogeneous exponential phase myceli
Autor:
Rolf Furter, Benjamin D. Hall
Publikováno v:
Plant Molecular Biology. 17:773-785
Wheat germ nuclear extracts inhibited an active yeast polymerase III (pol III) transcription extract. We isolated two chromatin-associated fractions which harbored biochemically distinguishable inhibitory activities, each contributing about 40–50%