Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Roland A Owens"'
Autor:
Kagnew H. Gebreyesus, Roland A. Owens
Publikováno v:
BioTechniques, Vol 34, Iss 3, Pp 512-522 (2003)
A difficulty that is encountered when attempting to insert a PCR-amplified product or DNA fragment of interest into a particular vector is the presence within the insert of one or more internal restriction endonuclease (RE) sites identical to those s
Externí odkaz:
https://doaj.org/article/36418c7254ea4431acd0d4d00eb7f8c5
Autor:
Roland A. Owens
Publikováno v:
Hum Gene Ther
In the 1980s and early 1990s, Dr. Barrie Carter served as the chief of the Laboratory of Molecular and Cellular Biology in the National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health. During that time, hi
Aims: Adeno-associated virus type 2 (AAV2) is a naturally defective human parvovirus that is being developed as a gene therapy vector. In dividing cells, AAV2 DNA persists by integration into the host chromosomes. AAV2 is unique among mammalian virus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2328ffb69514ffa77a84675b68697328
https://doi.org/10.7287/peerj.preprints.326v4
https://doi.org/10.7287/peerj.preprints.326v4
Aims: Adeno-associated virus type 2 (AAV2) is a naturally defective human parvovirus that is being developed as a gene therapy vector. In dividing cells, AAV2 DNA persists by integration into the host chromosomes. AAV2 is unique among mammalian virus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f53cabcfbc0e463c07e0fd460322b821
https://doi.org/10.7287/peerj.preprints.326v1
https://doi.org/10.7287/peerj.preprints.326v1
Publikováno v:
Archives of Biochemistry and Biophysics. 389:271-277
The strand-specific, site-specific endonuclease (nicking) activity of the Rep68 and Rep78 (Rep68/78) proteins of adeno-associated virus type 2 (AAV) is involved in AAV replication, and appears to be involved in AAV site-specific integration. Rep68/78
Publikováno v:
Journal of Virology. 74:2936-2942
The endonuclease activity of the Rep68 and Rep78 proteins (Rep68/78) of adeno-associated virus type 2 (AAV) cuts at the terminal resolution site ( trs ) within the hairpin structure formed by the AAV inverted terminal repeats. Recent studies suggest
Analysis of the Effects of Charge Cluster Mutations in Adeno-Associated Virus Rep68 Protein In Vitro
Publikováno v:
Journal of Virology. 73:2084-2093
Adeno-associated virus type 2 (AAV) is a human parvovirus with a single-stranded, linear DNA genome containing inverted terminal repeats which function as origins of replication (14, 40, 44, 53). AAV is nonpathogenic and usually requires an adenoviru
Publikováno v:
Virology. 236:167-176
The Rep68 and Rep78 proteins of adeno-associated virus type-2 (AAV) are multifunctional DNA binding proteins which are involved in the positive and negative regulation of AAV genes, as well as various cellular and heterologous viral genes. In this st
Publikováno v:
Journal of Virology. 71:2722-2730
The Rep68 and Rep78 proteins of adeno-associated virus type 2 (AAV) are multifunctional proteins which contain overlapping amino acid sequences. They are required for viral replication and preferential integration of the AAV genome into a region of h
Publikováno v:
Journal of Virology. 70:2440-2448
Both the Rep68 and Rep78 proteins of adeno-associated virus type 2 (AAV) bind to AAV terminal repeat hairpin DNA and can mediate site-specific nicking in vitro at the terminal resolution site (trs) within the terminal repeats. To define the regions o