Zobrazeno 1 - 10
of 232
pro vyhledávání: '"Roland, Contreras"'
Publikováno v:
BioTechniques, Vol 28, Iss 6, Pp 1112-1116 (2000)
Because Candida albicans is a diploid organism, two consecutive steps of gene disruption are required to generate a gene knock-out. The same marker (URA3) is often used for disruption of both copies of the gene. This is possible because, after the fi
Externí odkaz:
https://doaj.org/article/df712a7e72454101aeed26220852f709
Publikováno v:
Neoplasia: An International Journal for Oncology Research, Vol 1, Iss 6, Pp 557-567 (1999)
In addition to eugenetic changes, cancerous cells exhibit extensive modifications in the expression levels of a variety of genes. The phenotypic switch observed after inoculation of T lymphoma cells into syngenic mice illustrates the active participa
Externí odkaz:
https://doaj.org/article/497722b7cee4442faa8d12894f209704
Autor:
Charlot De Wachter, Katelijne Geysens, Paul Ameloot, Nico Callewaert, Bart Samyn, Roland Contreras, José Martins, Pieter P. Jacobs, Jurgen Haustraete, Jean-Christophe Renauld, Simon Devos, Bram Laukens, Willy Morelle
Publikováno v:
Biotechnology and bioengineering
Biotechnology and bioengineering, 2020, Biotechnology and bioengineering, 117 (8), pp.2479-2488. ⟨10.1002/bit.27375⟩
Biotechnology and bioengineering, 2020, Biotechnology and bioengineering, 117 (8), pp.2479-2488. ⟨10.1002/bit.27375⟩
BackgroundThe glycosylation pathways of several eukaryotic protein expression hosts are being engineered to enable the production of therapeutic glycoproteins with humanized application-customized glycan structures. In several expression hosts, this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b98af807cd471ca24cf4ebc4de82e33
https://doi.org/10.1101/2020.01.28.923631
https://doi.org/10.1101/2020.01.28.923631
Autor:
Roland Contreras, Xue-En Liu, Valerie Vanhooren, Cuiying Chitty Chen, Sylviane Dewaele, Jacques Van Huysse, Claude Libert, Ye-Dong Fan, Hui Zhuang, Ling Wang
Publikováno v:
Liver International. 30:1221-1228
Background and aims: There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5c719d2037a18c5de0f6b2add75478b5
https://doi.org/10.1111/j.1478-3231.2010.02279.x
https://doi.org/10.1111/j.1478-3231.2010.02279.x
Autor:
Qing Xie, Claude Libert, Sylviane Dewaele, Hui Wang, Cuiying Chen, Xue-En Liu, Chung-Fang Gao, Roland Contreras, Hui Zhuang, Honglian Gui, Ling Wang
Publikováno v:
Liver International. 30:259-267
Background: We previously reported on serum N-glycans as markers for the diagnosis of cirrhosis in patients with chronic hepatitis C infection. Our present study aimed to evaluate the use of serum glycan markers for the diagnosis of liver fibrosis in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75e2040cdbfbccc7fe3aeccd6c72c8e9
https://doi.org/10.1111/j.1478-3231.2009.02170.x
https://doi.org/10.1111/j.1478-3231.2009.02170.x
Publikováno v:
Nature Protocols. 4:58-70
Here we provide a protocol for engineering the N-glycosylation pathway of the yeast Pichia pastoris. The general strategy consists of the disruption of an endogenous glycosyltransferase gene (OCH1) and the stepwise introduction of heterologous glycos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9b896113d8df42826e7c14640136ca0
https://doi.org/10.1038/nprot.2008.213
https://doi.org/10.1038/nprot.2008.213
Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus fumigatus
Autor:
Gerhard H. Braus, Sven Krappmann, Armin Braun, Roland Contreras, Philipp S. Schmalhorst, Wouter Vervecken, Manfred Rohde, Meike Müller, Françoise H. Routier, Hans Bakker
Publikováno v:
EUKARYOTIC CELL
The filamentous fungus Aspergillus fumigatus is the primary cause of invasive aspergillosis, an often fatal condition affecting people with a weakened immune system. Along with the immunocompromised population, the incidence of invasive aspergillosis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::159aefb6f1f5a9c40c9c0ae8d41620a4
https://doi.org/10.1128/ec.00109-08
https://doi.org/10.1128/ec.00109-08
Autor:
Roland Contreras, Nico Callewaert, Sylviane Dewaele, Stefan Ryckaert, Pieter P. Jacobs, Isabelle Dewerte
Publikováno v:
Glycobiology. 18:137-144
The establishment of a robust technology platform for the expression cloning of carbohydrate-binding proteins remains a key challenge in glycomics. Here we explore the utility of using yeast surface display (YSD) technology in the interaction-based l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89121a02e37865c433b5840a2e53404f
https://doi.org/10.1093/glycob/cwm131
https://doi.org/10.1093/glycob/cwm131
Autor:
Rhj Begent, L Smith, RB Pedley, Berend Tolner, Roland Contreras, N Griffin, P Beckett, J Bhatia, Sk Sharma, T Hillyer, Wouter Vervecken, KA Chester, Lisa Robson
Publikováno v:
European Journal of Cancer. 43:2515-2522
Many promising recombinant cancer medicines are generated by academic research and increasing the number of these products that are translated into the clinic will increase the pipeline of new therapies. Recombinant proteins for use in Phase I/II can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::546925c1938d0ee115d1f13b47a9bf45
https://doi.org/10.1016/j.ejca.2007.08.027
https://doi.org/10.1016/j.ejca.2007.08.027
Autor:
Claude Libert, Wouter Laroy, Cuiying Chen, Chunfang Gao, Roland Contreras, Xue-En Liu, Valerie Vanhooren, Liesbeth Desmyter, Nico Callewaert, Hui Zhuang, Ling Wang, Sylviane Dewaele
Publikováno v:
Hepatology. 46:1426-1435
We evaluated the use of blood serum N-glycan fingerprinting as a tool for the diagnosis of hepatocellular carcinoma (HCC) in patients with cirrhosis induced by hepatitis B virus (HBV). A group of 450 HBV-infected patients with liver fibrosis or cirrh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f61a04e9ffe783963d3c273b6964bc66
https://doi.org/10.1002/hep.21855
https://doi.org/10.1002/hep.21855