An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

Autor: Julia Bittmann, Rokas Grigaitis, Lorenzo Galanti, Silas Amarell, Florian Wilfling, Joao Matos, Boris Pfander

Publikováno v: eLife, Vol 9 (2020)

Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein

Externí odkaz: https://doaj.org/article/f928617eaf154412bfc7142dc0ceeff1

Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion

Autor: Mindaugas Zaremba, Donata Dakineviciene, Edvardas Golovinas, Evelina Zagorskaitė, Edvinas Stankunas, Anna Lopatina, Rotem Sorek, Elena Manakova, Audrone Ruksenaite, Arunas Silanskas, Simonas Asmontas, Algirdas Grybauskas, Ugne Tylenyte, Edvinas Jurgelaitis, Rokas Grigaitis, Kęstutis Timinskas, Česlovas Venclovas, Virginijus Siksnys

Publikováno v: Nature microbiology 7(11), 1857-1869 (2022). doi:10.1038/s41564-022-01239-0

Nature microbiology 7(11), 1857 - 1869 (2022). doi:10.1038/s41564-022-01239-0
Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID and PIWI) and contribute to RN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be66bd03d24d2cf5ff56bbe4bca24e7
https://bib-pubdb1.desy.de/record/485697

The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis

Autor: Rokas Grigaitis, Paula Alonso-Ramos, Teresa Bermejo, David Álvarez-Melo, Carolina Pascual-Silva, Katerina Strouhalova, Meret Arter, Joao Matos, George B. Garside, Pedro A. San-Segundo, Jesús A. Carballo, Rahel Wettstein, Marta Fernández-Díaz, Marco Geymonat

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 9811, p 9811 (2021)
International Journal of Molecular Sciences, 22 (18)
Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences
Volume 22
Issue 18

Meiotic defects derived from incorrect DNA repair during gametogenesis can lead to mutations, aneuploidies and infertility. The coordinated resolution of meiotic recombination intermediates is required for crossover formation, ultimately necessary fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45cc3863d969ad60771a2f0b7c582586
https://www.mdpi.com/1422-0067/22/18/9811

Author response: An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

Autor: Joao Matos, Rokas Grigaitis, Boris Pfander, Florian Wilfling, Silas Amarell, Lorenzo Galanti, Julia Bittmann

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e77e7dbcb825cc22c7681ca907cd855f
https://doi.org/10.7554/elife.52459.sa2

Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis

Autor: Petr Cejka, Ilaria Ceppi, Matthias Peter, Adrian Henggeler, Kristina Kasaciunaite, Aitor Susperregui, Joao Matos, Philipp Wild, Lepakshi Ranjha, Rokas Grigaitis, Vera M. Kissling, Ralf Seidel

Publikováno v: Developmental Cell, 53 (6)
Developmental Cell

Summary The Bloom’s helicase ortholog, Sgs1, orchestrates the formation and disengagement of recombination intermediates to enable controlled crossing-over during meiotic and mitotic DNA repair. Whether its enzymatic activity is temporally regulate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9684f5908315f829719effff907f1cc5
https://doi.org/10.1016/j.devcel.2020.05.016