Activation of recombinases at specific DNA loci by zinc-finger domain insertions.

Autor: Mukhametzyanova L; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany., Schmitt LT; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany.; Seamless Therapeutics GmbH, Dresden, Germany., Torres-Rivera J; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany., Rojo-Romanos T; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany.; Seamless Therapeutics GmbH, Dresden, Germany., Lansing F; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany.; Seamless Therapeutics GmbH, Dresden, Germany., Paszkowski-Rogacz M; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany., Hollak H; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany.; Seamless Therapeutics GmbH, Dresden, Germany., Brux M; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany., Augsburg M; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany., Schneider PM; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany.; Seamless Therapeutics GmbH, Dresden, Germany., Buchholz F; Medical Systems Biology, Medical Faculty, Technical University Dresden, Dresden, Germany. frank.buchholz@tu-dresden.de.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2024 Dec; Vol. 42 (12), pp. 1844-1854. Date of Electronic Publication: 2024 Jan 31.

Correction of a Factor VIII genomic inversion with designer-recombinases

Autor: Maciej Paszkowski-Rogacz, Frank Buchholz, Lukas Theo Schmitt, Takanori Takebe, Kentaro Iwasawa, Hoersten J, Masaki Kimura, Felix Lansing, Janet Karpinski, Rodriguez-Muela N, Grass T, Sonntag J, Günes C, Schneider Pm, Knöfler R, Rojo-Romanos T, Mukhametzyanova L

Despite advances in nuclease-based genome editing technologies, correcting human disease-causing genomic inversions remains a challenge. Here, we describe the potential use of a recombinase-based system to correct a 140 kb genomic inversion of the F8

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::043cd7d69c0f8c93fef44dd597cfdbfc
https://doi.org/10.1101/2020.11.02.328013

Correction of a Factor VIII genomic inversion with designer-recombinases.

Autor: Lansing F; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Mukhametzyanova L; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Rojo-Romanos T; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Iwasawa K; Division of Gastroenterology, Hepatology and Nutrition, Division of Developmental Biology, Center for Stem Cell and Organoid Medicine (CuSTOM) Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA., Kimura M; Division of Gastroenterology, Hepatology and Nutrition, Division of Developmental Biology, Center for Stem Cell and Organoid Medicine (CuSTOM) Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Paszkowski-Rogacz M; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Karpinski J; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Grass T; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany.; German Center for Neurodegenerative Diseases, Helmholtz Association, 01307, Dresden, Germany., Sonntag J; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Schneider PM; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Günes C; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster, 48149, Germany., Hoersten J; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Schmitt LT; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany., Rodriguez-Muela N; German Center for Neurodegenerative Diseases, Helmholtz Association, 01307, Dresden, Germany., Knöfler R; Department of Pediatric Hematology and Oncology, University Hospital Dresden, 01307, Dresden, Germany., Takebe T; Division of Gastroenterology, Hepatology and Nutrition, Division of Developmental Biology, Center for Stem Cell and Organoid Medicine (CuSTOM) Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.; Institute of Research, Tokyo Medical and Dental University (TMDU), Tokyo, Japan., Buchholz F; Medical Systems Biology, Medical Faculty, Technical University Dresden, 01307, Dresden, Germany. frank.buchholz@tu-dresden.de.

Publikováno v: Nature communications [Nat Commun] 2022 Jan 20; Vol. 13 (1), pp. 422. Date of Electronic Publication: 2022 Jan 20.

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus.

Autor: Lansing F; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Paszkowski-Rogacz M; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Schmitt LT; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Schneider PM; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Rojo Romanos T; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Sonntag J; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany., Buchholz F; Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2020 Jan 10; Vol. 48 (1), pp. 472-485.

Behavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans .

Autor: Rojo Romanos T; Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia.; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark., Ng L; Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Pocock R; Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia.; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.

Publikováno v: Bio-protocol [Bio Protoc] 2018 Jan 05; Vol. 8 (1).