Zobrazeno 1 - 10
of 97
pro vyhledávání: '"Roger L. P. Adams"'
Publikováno v:
Insect Biochemistry and Molecular Biology. 33:681-689
When cultured insect cells (Sf9) were grown in the presence of 5 x 10(-6) M azadirachtin, there was a rapid increase in the mitotic index, with the appearance of many aberrant mitotic figures. Flow cytometry established that cells accumulated in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fdf71a06bea158c163ae5353a07f431
https://doi.org/10.1016/s0965-1748(03)00057-2
https://doi.org/10.1016/s0965-1748(03)00057-2
Publikováno v:
Biochemical Journal. 341:473-476
The presence of two DNA methyltransferases in Pisum raises the possibility that they serve different functions. In vitro methylation of CWG sequences in the strong cauliflower mosaic virus 35S promoter had no effect on reporter gene expression. In co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2e7030c99842825759b89c0e498d49d
https://doi.org/10.1042/bj3410473
https://doi.org/10.1042/bj3410473
Publikováno v:
Molecular Biology Reports. 26:261-267
Lipofection, a lipid-mediated DNA transfection procedure, was used to transfect synchronized L929 mouse fibroblast cells with a reporter plasmid containing the bacterial chloramphenicol acetyltransferase gene. The efficiency of gene expression was in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::09103ca4bd0e95c53bbabeb85afc9171
https://doi.org/10.1023/a:1007009022336
https://doi.org/10.1023/a:1007009022336
Autor:
Roger L. P. Adams
Publikováno v:
BioEssays. 17:139-145
Methylation of DNA plays an important role in the control of gene expression in higher eukaryotes. This is largely achieved by the packaging of methylated DNA into chromatin structures that are inaccessible to transcription factors and other proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55c1a79f54f3d08877f3fc7a332f2109
https://doi.org/10.1002/bies.950170209
https://doi.org/10.1002/bies.950170209
Publikováno v:
Biochemical Journal. 305:791-798
We have previously shown that DNA methylation acts as a focus for the formation of inactive chromatin in vivo. We have investigated the mechanism further by in vitro transcription of a template containing two tRNA genes and an extensive (G+C)-rich se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6be312a6088484949e4c6bd544a413cf
https://doi.org/10.1042/bj3050791
https://doi.org/10.1042/bj3050791
Autor:
Sriharsa Pradhan, Roger L. P. Adams, Claudio Passananti, Paola Caiafa, Anna Reale, Giuseppe Zardo, Serena Buontempo, Giovanna De Matteis
Publikováno v:
16 (2002): 1319–1321.
info:cnr-pdr/source/autori:Zardo G. 1, Reale A. 1, Passananti C. 2, Pradhan S. 3, Buontempo S.1, De Matteis G. 1, Adams R.L.P. 4, and Caiafa P.1/titolo:Inhibition of poly(ADP-ribosyl)ation induces DNA hypermethylation: a possible molecular mechanism/doi:/rivista:/anno:2002/pagina_da:1319/pagina_a:1321/intervallo_pagine:1319–1321/volume:16
Scopus-Elsevier
info:cnr-pdr/source/autori:Zardo G. 1, Reale A. 1, Passananti C. 2, Pradhan S. 3, Buontempo S.1, De Matteis G. 1, Adams R.L.P. 4, and Caiafa P.1/titolo:Inhibition of poly(ADP-ribosyl)ation induces DNA hypermethylation: a possible molecular mechanism/doi:/rivista:/anno:2002/pagina_da:1319/pagina_a:1321/intervallo_pagine:1319–1321/volume:16
Scopus-Elsevier
The pattern of DNA methylation established during embryonic development is necessary for the control of gene expression and is preserved during the replicative process. DNA regions of about 1-2 kb in size, termed CpG islands and located mostly in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7babcc30ae69ad96093bdf9529cdb710
https://doi.org/10.1096/fj.01-0827fje
https://doi.org/10.1096/fj.01-0827fje
Publikováno v:
Biochemical Journal. 293:617-624
DNA methylase, present in low-salt extracts of nuclei prepared from Pisum sativum shoot tips, methylates model DNA substrates containing CNG trinucleotides or CI dinucleotides only. The binding to the hemimethylated trinucleotide substrates is very m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61670ec44bd61a8a82e3d752563a1e91
https://doi.org/10.1042/bj2930617
https://doi.org/10.1042/bj2930617
Publikováno v:
Philosophical Transactions of the Royal Society of London. B, Biological Sciences. 326:189-198
DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd5fe995d51d97f53208c842a3b48949
https://doi.org/10.1098/rstb.1990.0003
https://doi.org/10.1098/rstb.1990.0003
Publikováno v:
Gene. 157:289-291
We have purified two distinct DNA methyltransferases from pea shoot tips and analysed their sequence specificity using synthetic oligodeoxyribonucleotide substrates and chemical sequencing methods. One methylates only CG target sequences, whereas the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::266ef41c424022bc2e8a6e6cd43095db
https://doi.org/10.1016/0378-1119(95)00076-i
https://doi.org/10.1016/0378-1119(95)00076-i
Publikováno v:
Genomic Imprinting ISBN: 9780896037410
DNA methylation at position 5 in the cytosine ring in the sequence CpG can be detrimental to the transcription of a variety of genes in higher eukaryotes (1,2). Although the significance of this transcriptional repression is currently under debate (3
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9f6308bb0d80d5de172e692eaaa4d80f
https://doi.org/10.1385/1-59259-211-2:243
https://doi.org/10.1385/1-59259-211-2:243
