Cysteine residues of Carnation Italian Ringspot virus p19 suppressor of RNA silencing maintain global structural integrity and stability for siRNA binding

Autor: Roger Koukiekolo, Katarzyna Kieliszkiewicz, Jenny Cheng, John Paul Pezacki, Selena M. Sagan

Publikováno v: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1794:1197-1203

Carnation Italian Ringspot virus (CIRV) has evolved a protein called p19 that acts as a suppressor of RNA silencing in the host cell and aids in viral persistence. This protein has been shown to be sensitive to cysteine alkylation resulting in a redu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5bab520602ae9e7951c2e644ae8151c
https://doi.org/10.1016/j.bbapap.2009.03.012

Porcine pancreatic α-amylase inhibition by the kidney bean (Phaseolus vulgaris) inhibitor (α-AI1) and structural changes in the α-amylase inhibitor complex

Autor: Roger Koukiekolo, Guy Marchis-Mouren, Marius Santimone, Véronique Le Berre, Véronique Desseaux, Pierre Rougé, Yann Moreau

Publikováno v: Biochimica et Biophysica Acta Proteins and Proteomics
Biochimica et Biophysica Acta Proteins and Proteomics, Elsevier, 2004, 1696 (2), pp.181-190. ⟨10.1016/j.bbapap.2003.11.001⟩
Biochimica et Biophysica Acta Proteins and Proteomics, 2004, 1696 (2), pp.181-190. ⟨10.1016/j.bbapap.2003.11.001⟩

Porcine pancreatic alpha-amylase (PPA) is inhibited by the red kidney bean (Phaseolus vulgaris) inhibitor alpha-AI1 [Eur. J. Biochem. 265 (1999) 20]. Inhibition kinetics were carried out using DP 4900-amylose and maltopentaose as substrate. As shown

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc40e7d1ab229745a24f02ca3d5e4b47
https://doi.org/10.1016/j.bbapap.2003.11.001

Starch digestion in tropical fishes: isolation, structural studies and inhibition kinetics of α-amylases from two tilapias Oreochromis niloticus and Sarotherodon melanotheron

Autor: Marius Santimone, Guy Marchis-Mouren, Roger Koukiekolo, Véronique Desseaux, Yann Moreau

Publikováno v: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 128:543-552

alpha-Amylases from the intestinal cavity of two tilapia species, Oreochromis niloticus (ONI-AMY) and Sarotherodon melanotheron (SME-AMY), were purified using ammonium sulfate precipitation, affinity chromatography and chromatofocusing procedures. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::278e0588a44dc3c56095f314bf4e4377
https://doi.org/10.1016/s1096-4959(00)00358-4

Studies of a viral suppressor of RNA silencing p19-CFP fusion protein: a FRET-based probe for sensing double-stranded fluorophore tagged small RNAs

Autor: Zygmunt J. Jakubek, Selena M. Sagan, John Paul Pezacki, Roger Koukiekolo, Jenny Cheng

Publikováno v: Biophysical chemistry. 143(3)

Eukaryotes have evolved complex cellular responses to double-stranded RNA. One response that is highly conserved across many species is the RNA silencing pathway. Tombusviruses have evolved a mechanism to evade the RNA silencing pathway that involves

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20f8d8460e34bb8524470f1e8fd5bdbb
https://pubmed.ncbi.nlm.nih.gov/19491057

Stabilized recombinant suppressors of RNA silencing: functional effects of linking monomers of Carnation Italian Ringspot virus p19

Autor: John Paul Pezacki, Jenny Cheng, Natalie K. Goto, Naila Assem, Roger Koukiekolo, Selena M. Sagan

Eukaryotes have evolved complex cellular responses to double-stranded RNA including the RNA silencing pathway. Tombusviruses have adapted a mechanism to evade RNA silencing that involves a 19 kDa dimeric protein (p19) that is a suppressor of RNA sile

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2635ae82f318e55fc337746d2c6f8221
https://doi.org/10.1016/j.bbapap.2007.09.014