Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Roger J. Kemble"'
Publikováno v:
Plant Cell Reports. 9
Protoplast fusion was utilised to transfer Polima type cytoplasmic male sterility (CMS) from Brassica napus, canola cv. Polima Karat (Pol-Karat) to B. oleracea, broccoli, var. "Green Comet". Southern and RFLP analysis confirmed that four cybrids poss
Autor:
John E. Carlson, Roger J. Kemble
Publikováno v:
Plant Molecular Biology. 4:117-123
Mitochondrial DNA (mtDNA) was isolated from over 100 different maize nucleo-cytoplasmic combinations. DNA preparations were assayed for the presence of the 1.94kb mitochondrial plasmid by agarose gel electrophoresis and hybridization to a recombinant
Publikováno v:
Genetics. 93:189-200
The genetic control of major wheat endosperm proteins by homoeologous group 1 chromosomes has been studied by two-dimensional polyacrylamide gel electrophoresis. The control of at least 15 distinct protein subunits or groups of protein subunits has b
Publikováno v:
Plant Science. 53:243-248
‘Polima’ cytoplasmic male sterility (cms) was transferred in one step from spring to winter lines of oilseed rape (Brassica. napus L.) by protoplast fusion. The transfer was confirmed by restriction fragment length polymorphism (RFLP) analysis of
Publikováno v:
Molecular and General Genetics MGG. 213:202-205
Brassica napus cybrid plants which contain novel nucleus-mitochondria-chloroplast combinations have been constructed, via protoplast fusion. Such fusions resulted in mitochondrial DNA plasmids being lost (at a frequency of 12.5%) or, more surprisingl
Publikováno v:
Science. 219:683-688
Protoplasts of sexually incompatible species have been fused and in some combinations have given rise to somatic hybrid plants. Partial elimination of parental chromosomes from either species is common in such hybrids, but total chromosome loss has g
Autor:
Roger J. Kemble, Gerry L. Brown
Publikováno v:
Current Genetics. 15:341-347
An in vitro complex capable of supporting DNA replication was isolated from Brassica L. mitochondria. Template preferences and inhibitor studies confirmed τ DNA polymerase characteristics. Comparison of complexes isolated from B. napus, B. campestri
Publikováno v:
Nature. 304:744-747
The endosymbiont hypothesis for the origin of eukaryotic cells implies that an exchange of genetic information between nuclei, mitochondria and, in the case of plants, chloroplasts is plausible. Indeed, a 12-kilobase (kb) DNA sequence common to chlor
Autor:
Tina L. Barsby, Roger J. Kemble
Publikováno v:
Biochemistry and Cell Biology. 66:665-676
Protoplast fusion can overcome the sexual incompatibility barriers that exist between different plant species and can give rise to novel plants that cannot be produced in nature. It therefore provides a new source of genetic variation, particularly w
Publikováno v:
Current Genetics. 11:151-160
An in organello system is described in which radioactive nucleoside triphosphates are linearly incorporated into maize mitochondrial DNA and RNA for more than 60 minutes at 37 °C. The nuclear control over plasmid-like mitochondrial DNAs observed in