Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Roger J P Dawson"'
Autor:
Shoji Maeda, Dawei Sun, Ankita Singhal, Marcello Foggetta, Georg Schmid, Joerg Standfuss, Michael Hennig, Roger J P Dawson, Dmitry B Veprintsev, Gebhard F X Schertler
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e98714 (2014)
The activation of the G-protein transducin (Gt) by rhodopsin (Rho) has been intensively studied for several decades. It is the best understood example of GPCR activation mechanism and serves as a template for other GPCRs. The structure of the Rho/G p
Externí odkaz:
https://doaj.org/article/8b5da6ab8ec342a3876cec257f4820f5
Autor:
Shoji Maeda, Antoine Koehl, Hugues Matile, Hongli Hu, Daniel Hilger, Gebhard F. X. Schertler, Aashish Manglik, Georgios Skiniotis, Roger J. P. Dawson, Brian K. Kobilka
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
The determination of high resolution structures of G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins is challenging. Here authors develop an antibody fragment, mAB16, which stabilizes GPCR/G-protein complexes and facilitat
Externí odkaz:
https://doaj.org/article/78767849a46d4536a969df36dd817784
Autor:
Roger J. P. Dawson, Emmanuel Pinard, Poul Nissen, Gleb Bourenkov, Wolfgang Guba, Azadeh Shahsavar, Peter Stohler, Thomas R. Schneider, Markus A. Seeger, Martin Siegrist, Iwan Zimmermann
The human glycine transporter 1 (GlyT1) regulates glycine mediated neuronal excitation and inhibition through sodium- and chloride-dependent reuptake of the neurotransmitter1-3. Inhibition of glycine reuptake via GlyT1 prolongs neurotransmitter signa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::510a4015d88ead692ea0fc106d6c1255
https://doi.org/10.1101/2020.12.20.110478
https://doi.org/10.1101/2020.12.20.110478
Autor:
Wolfgang Guba, Markus A. Seeger, Azadeh Shahsavar, Iwan Zimmermann, Thomas R. Schneider, Steffen Sinning, Gleb Bourenkov, Emmanuel Pinard, Poul Nissen, Peter Stohler, Martin Siegrist, Roger J. P. Dawson
Publikováno v:
Shahsavar, A, Stohler, P, Bourenkov, G, Zimmermann, I, Siegrist, M, Guba, W, Pinard, E, Sinning, S, Seeger, M A, Schneider, T R, Dawson, R J P & Nissen, P 2021, ' Structural insights into the inhibition of glycine reuptake ', Nature, vol. 591, pp. 677-681 . https://doi.org/10.1038/s41586-021-03274-z
The human glycine transporter 1 (GlyT1) regulates glycine-mediated neuronal excitation and inhibition through the sodium- and chloride-dependent reuptake of glycine1–3. Inhibition of GlyT1 prolongs neurotransmitter signalling, and has long been a k
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5fbb4ec10167844f6037a4d2725e07
https://pubmed.ncbi.nlm.nih.gov/33658720
https://pubmed.ncbi.nlm.nih.gov/33658720
Autor:
Philipp Bräuer, Pascal Egloff, Simon Newstead, Markus A. Seeger, Eric R. Geertsma, Benedikt T Kuhn, Roger J. P. Dawson, Iwan Zimmermann, Cedric A. J. Hutter
Here, we provide a protocol to generate synthetic nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week period. Unlike methods that require animals for antibody generation, sybody selections are carried out en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60fb504f98721c103baafee2d8c5815
https://doi.org/10.5167/uzh-190529
https://doi.org/10.5167/uzh-190529
Autor:
Benedikt T, Kuhn, Iwan, Zimmermann, Pascal, Egloff, Lea M, Hürlimann, Cedric A J, Hutter, Christian, Miscenic, Roger J P, Dawson, Markus A, Seeger, Eric R, Geertsma
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2127
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5668c4a74b9001ba57345ae5cb11be27
https://pubmed.ncbi.nlm.nih.gov/32112321
https://pubmed.ncbi.nlm.nih.gov/32112321
Autor:
Peter, Stohler, Roger J P, Dawson
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2127
Over the last decades, the use of heavy-chain-only antibodies has received growing attention in academia and industry as research and diagnostic tools as well as therapeutics. Their generation has improved with the help of innovative new methods such
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d4c3f046a63396698423a5ed5b34e237
https://pubmed.ncbi.nlm.nih.gov/32112323
https://pubmed.ncbi.nlm.nih.gov/32112323
Autor:
Roger J. P. Dawson, Markus A. Seeger, Pascal Egloff, Eric R. Geertsma, Iwan Zimmermann, Cedric A. J. Hutter, Christian Miscenic, Benedikt T Kuhn, Lea M. Hürlimann
Publikováno v:
Methods in Molecular Biology ISBN: 9781071603727
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b424f3c68e9441391fb7b81386de0428
https://www.zora.uzh.ch/id/eprint/196535/
https://www.zora.uzh.ch/id/eprint/196535/
Autor:
Peter Stohler, Roger J. P. Dawson
Publikováno v:
Methods in Molecular Biology ISBN: 9781071603727
Over the last decades, the use of heavy-chain-only antibodies has received growing attention in academia and industry as research and diagnostic tools as well as therapeutics. Their generation has improved with the help of innovative new methods such
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e3c08c435cfb303d82f0d6eaef79838
https://doi.org/10.1007/978-1-0716-0373-4_13
https://doi.org/10.1007/978-1-0716-0373-4_13
Publikováno v:
BIOspektrum. 24:688-690
The mechanistic characterization of membrane proteins requires their stabilization in specific conformations. Camelid single-domain antibodies are particularly suited for this purpose due to their unconventional design. However, their broad deployabi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::47f1e13d0ce80fce592e273d413a5533
https://doi.org/10.1007/s12268-018-0982-x
https://doi.org/10.1007/s12268-018-0982-x