Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Roger E. Calza"'
Autor:
Roger E. Calza, R. John Wallace, Freda M. McIntosh, Sylvain C. P. Eschenlauer, Neil R. McEwan, C. Jamie Newbold, Derek Gatherer
Publikováno v:
Anaerobe. 6:21-28
A bias in the codon utilization pattern of members of the ciliate family Ophryoscolecidae is described. A pattern of preferential use of AGA to encode arginine, and a bias against the occurrence of G in position three of codons for lysine, glutamine
Publikováno v:
Protist. 150:257-264
The phylogenetic position of eleven 14-3-3 proteins from five protozoal species was tested relative to other eukaryotic 14-3-3 versions representing many of the previously described isoforms. The protozoal proteins, four from Entodinium caudatum, thr
Publikováno v:
Applied Microbiology and Biotechnology. 45:811-816
Aspergillus oryzae fermentation extract (Amaferm) was used to stimulate the in vitro growth of the cellulolytic fungus Neocallimastix frontalis EB 188. Soluble and filter-sterilized extract was added either at the start or throughout culture growth.
Publikováno v:
Applied Microbiology and Biotechnology. 45:817-821
Three fungi Neocallimastix frontalis EB 188, Piromyces communis DC 193 and Orpinomyces ssp. RW 206, representing the predominant cultures isolated from cattle, were shown to respond to the addition of Aspergillus oryzae fermentation extract (i.e., Am
Autor:
Roger E. Calza, Kwang Pin Tsai
Publikováno v:
Applied Microbiology and Biotechnology. 39:477-482
Variations in culture feeding protocols were used to optimize the secretion of protein and cellulase in Neocallimastix frontalis EB188. High numbers (2000/ml) of zoospores, culture feeding at 55 h using a 1:3 dilution and cotton cellulose [0.25% (w/v
Autor:
Roger E. Calza
Publikováno v:
Current Microbiology. 23:175-180
De novo synthesis and the secretion of cellobiase fromNeocallimastix frontalis EB188 were studied. Cellobiase was secreted rapidly in cellulose switch cultures. Chemical inhibition of protein synthesis and radiotracer studies suggested secretion was
Autor:
Xinliang Li, Roger E. Calza
Publikováno v:
Enzyme and Microbial Technology. 13:622-628
An extracellular β-glucosidase (E3.2.1.21) from the rumen fungus Neocallimastix frontalis EB188 was purified. The purification protocol included hydroxylapatite, gel filtration, and ion exchange chromatographies and native polyacrylamide gel electro
Autor:
Roger E. Calza
Publikováno v:
Current Microbiology. 22:213-219
Protein and cellulase secretion inNeocallimastix frontalis EB188 was studied. Induction of secretion in this ruminal fungus was dependent on the amount of medium cellulose and on de novo protein synthesis. Medium glucose repressed secretions and dimi
Autor:
Roger E. Calza
Publikováno v:
Current Microbiology. 21:109-115
Extracellular protein and cellulase excretion secretions were studied in the ruminal fungusNeocallimastix frontalis EB188. Cellulase assay and polyacrylamide gel electrophoresis was used to characterize protein excretion patterns caused by media swit
Autor:
E. M. Barichievich, Roger E. Calza
Publikováno v:
Current Microbiology. 20:265-271
Extracellular cellulase induction in the ruminal fungusNeocallimastix frontalis isolate EB188 was followed. Glucose media-established cultures produced cellulase when switched to a variety of cellulose-containing media. High levels of cellulase and x