Transcriptional regulation is insufficient to explain substrate‐induced flux changes in Bacillus subtilis

Autor: Victor Chubukov, Markus Uhr, Ludovic Le Chat, Roelco J Kleijn, Matthieu Jules, Hannes Link, Stephane Aymerich, Jörg Stelling, Uwe Sauer

Publikováno v: Molecular Systems Biology, Vol 9, Iss 1, Pp 1-13 (2013)

Abstract One of the key ways in which microbes are thought to regulate their metabolism is by modulating the availability of enzymes through transcriptional regulation. However, the limited success of efforts to manipulate metabolic fluxes by rewirin

Externí odkaz: https://doaj.org/article/c50f2e99c3d44e0ea17aeb1ecf3bc065

Regulatory Tasks of the Phosphoenolpyruvate-Phosphotransferase System of Pseudomonas putida in Central Carbon Metabolism

Autor: Max Chavarría, Roelco J. Kleijn, Uwe Sauer, Katharina Pflüger-Grau, Víctor de Lorenzo

Publikováno v: mBio, Vol 3, Iss 2 (2012)

ABSTRACT Two branches of the phosphoenolpyruvate-phosphotransferase system (PTS) operate in the soil bacterium Pseudomonas putida KT2440. One branch encompasses a complete set of enzymes for fructose intake (PTSFru), while the other (N-related PTS, o

Externí odkaz: https://doaj.org/article/3360aa076889444ab08afb70c211d31d

Global Network Reorganization During Dynamic Adaptations of Bacillus subtilis Metabolism

Autor: Claus Hultschig, Patrick Veiga, Edda Klipp, Michael Hecker, Annette Hansen, Etienne Dervyn, Elena Bidnenko, Philippe Noirot, Bernd Hessling, Emma L. Denham, Markus Uhr, Susanne Pohl, Ludovic Le Chat, Joerg Martin Buescher, Benno Schwikowski, Hanne Østergaard Jarmer, Jan Muntel, Bernd Rinn, Pierre Nicolas, Jan Maarten van Dijl, Roelco J. Kleijn, Simon Rasmussen, Colin R. Harwood, Samuel Drulhe, Liza Felicori, Jörg Stelling, Sean Walsh, Kevin M. Devine, Frank Rügheimer, Nathalie Pigeonneau, Dörte Becher, Mark J. Fogg, Peter J. Lewis, Eric Botella, Geoff Doherty, Philippe Bessières, Ulrike Mäder, Marc Schaffer, Sjouke Piersma, Stéphane Aymerich, Anthony J. Wilkinson, Anne Goelzer, Julian Schnidder, F. Molina, Wolfram Liebermeister, Uwe Sauer, Sabine Peres, Sebastian Hubner, Matthieu Jules, François Lecointe, Aurélie Leduc

Publikováno v: Science
Science, American Association for the Advancement of Science, 2012, 335 (6072), pp.1099-1103. ⟨10.1126/science.1206871⟩
Science, 2012, 335 (6072), pp. 1099-1103. ⟨10.1126/science.1206871⟩
Science, 2012, 335 (6072), pp.1099-1103. ⟨10.1126/science.1206871⟩
Science, 335(6072), 1099-1103. AMER ASSOC ADVANCEMENT SCIENCE

Outside In Acquisition and analysis of large data sets promises to move us toward a greater understanding of the mechanisms by which biological systems are dynamically regulated to respond to external cues. Now, two papers explore the responses of a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec31dfbe5e60ff7fce5721e46e4e5360
https://research.rug.nl/en/publications/43e8904b-4689-4bfe-aa20-09645cc7a615

Metabolic flux analysis of a glycerol-overproducingSaccharomyces cerevisiaestrain based on GC-MS, LC-MS and NMR-derived13C-labelling data

Autor: Jack T. Pronk, Jan-Maarten A. Geertman, Antonius J. A. van Maris, Wouter A. van Winden, Dick Schipper, Cor Ras, Joseph J. Heijnen, Roelco J. Kleijn, Beckley K. Nfor

Publikováno v: FEMS Yeast Research. 7:216-231

This study focuses on unravelling the carbon and redox metabolism of a previously developed glycerol-overproducing Saccharomyces cerevisiae strain with deletions in the structural genes encoding triosephosphate isomerase ( TPI1 ), the external mitoch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7a2cc58f35bdc5a44613de87118658e1
https://doi.org/10.1111/j.1567-1364.2006.00180.x

13 C-Labeled Gluconate Tracing as a Direct and Accurate Method for Determining the Pentose Phosphate Pathway Split Ratio in Penicillium chrysogenum

Autor: Joseph J. Heijnen, Cor Ras, Walter M. van Gulik, Roelco J. Kleijn, Wouter A. van Winden, Dick Schipper

Publikováno v: Applied and Environmental Microbiology. 72:4743-4754

In this study we developed a new method for accurately determining the pentose phosphate pathway (PPP) split ratio, an important metabolic parameter in the primary metabolism of a cell. This method is based on simultaneous feeding of unlabeled glucos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6eacad8c3612531fed3b1dff7742624
https://doi.org/10.1128/aem.02955-05