Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Roee, Amit"'
Autor:
Inbal Vaknin, Or Willinger, Jonathan Mandl, Hadar Heuberger, Dan Ben-Ami, Yi Zeng, Sarah Goldberg, Yaron Orenstein, Roee Amit
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract We demonstrate a transcriptional regulatory design algorithm that can boost expression in yeast and mammalian cell lines. The system consists of a simplified transcriptional architecture composed of a minimal core promoter and a synthetic up
Externí odkaz:
https://doaj.org/article/86a25de504a84dddba6f03abb43c97a9
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Condensates composed of RNA and proteins are biologically vital but generally poorly understood. Here, the authors engineer synthetic RNA-protein condensates and show that gel-like condensates form as a result of liquid-gel phase separation in a spec
Externí odkaz:
https://doaj.org/article/e5bb6096b311492faa16a08bdc844edf
Autor:
Noa Katz, Eitamar Tripto, Naor Granik, Sarah Goldberg, Orna Atar, Zohar Yakhini, Yaron Orenstein, Roee Amit
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Phage-coat proteins can be used to build synthetic biology parts. Here the authors use an oligo library and machine learning to predict and verify sequences based on binding scores.
Externí odkaz:
https://doaj.org/article/9616667022114a1680b5c585955aa3b8
Autor:
Lior Levy, Leon Anavy, Oz Solomon, Roni Cohen, Michal Brunwasser-Meirom, Shilo Ohayon, Orna Atar, Sarah Goldberg, Zohar Yakhini, Roee Amit
Publikováno v:
Cell Reports, Vol 21, Iss 3, Pp 845-858 (2017)
We use an oligonucleotide library of >10,000 variants to identify an insulation mechanism encoded within a subset of σ54 promoters. Insulation manifests itself as reduced protein expression for a downstream gene that is expressed by transcriptional
Externí odkaz:
https://doaj.org/article/7d4fd9ada4074558bd5319622a2cf784
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
Some repressors down-regulate gene expression when they bind to a site up to 100 base pairs away from the nearest activator. Here, the authors show that this quenching-like repression can be achieved by the excluded volume effect when a DNA-bound pro
Externí odkaz:
https://doaj.org/article/557097a60f26479c91535bc7e20b92af
Autor:
Nanami Kikuchi, Sarah Goldberg, Beate Kaufmann, Lisa Kermas, Roee Amit, Or Willinger, Noa Navon
Publikováno v:
ACS Synthetic Biology. 10:1808-1820
We present Triplex-seq, a deep-sequencing method that systematically maps the interaction space between an oligo library of ssDNA triplex-forming oligos (TFOs) and a particular dsDNA triplex target site (TTS). We demonstrate the method using a random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3913f37af0dea5e3c35c66d8c7dee826
https://doi.org/10.1021/acssynbio.1c00122
https://doi.org/10.1021/acssynbio.1c00122
Publikováno v:
PLoS Computational Biology, Vol 13, Iss 1, p e1005337 (2017)
We model the regulatory role of proteins bound to looped DNA using a simulation in which dsDNA is represented as a self-avoiding chain, and proteins as spherical protrusions. We simulate long self-avoiding chains using a sequential importance samplin
Externí odkaz:
https://doaj.org/article/0cb8eed776ab4e49ae70b256db6eeef4
Autor:
Nanami Kikuchi, Or Willinger, Naor Granik, Reut Gal, Noa Navon, Shanny Ackerman, Ella Samuel, Tomer Antman, Noa Katz, Sarah Goldberg, Roee Amit
Publikováno v:
ACS synthetic biology. 11(4)
We present a cell-free assay for rapid screening of candidate inhibitors of protein binding, focusing on inhibition of the interaction between the SARS-CoV-2 Spike receptor binding domain (RBD) and human angiotensin-converting enzyme 2 (hACE2). The a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbca629d91ff5d4c8999b5f5d91081f8
https://pubmed.ncbi.nlm.nih.gov/35377616
https://pubmed.ncbi.nlm.nih.gov/35377616
Autor:
Nanami Kikuchi, Or Willinger, Noa Navon, Ella Samuel, Sarah Goldberg, Shanny Ackerman, Roee Amit, Naor Granik, Noa Katz, Tomer Antman
We present a cell-free assay for rapid screening of candidate inhibitors of protein binding, focusing on inhibition of the interaction between the SARS-CoV-2 Spike receptor binding domain (RBD) and human angiotensin-converting enzyme 2 (hACE2). The a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::61cae1c8743814c021fc6786f33682bb
https://doi.org/10.1101/2021.10.06.462907
https://doi.org/10.1101/2021.10.06.462907
