Characterisation of kinetics, substrate inhibition and product activation by AMP of bifunctional ADP ‐dependent glucokinase/phosphofructokinase from Methanococcus maripaludis

Autor: Gabriel Vallejos‐Baccelliere, Sergio B. Kaufman, Rodolfo M. González‐Lebrero, Víctor Castro‐Fernandez, Victoria Guixé

Publikováno v: The FEBS Journal. 289:7519-7536

Methanogenic archaea have received attention due to their potential use in biotechnological applications such as methane production, so their metabolism and regulation are topics of special interest. When growing in a nutrient-rich medium, these orga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43a8277fe8c13a3f4bc6052d36255d9e
https://doi.org/10.1111/febs.16557

Steady-state NTPase activity of Dengue virus NS3: number of catalytic sites, nucleotide specificity and activation by ssRNA.

Autor: J Jeremías Incicco, Leopoldo G Gebhard, Rodolfo M González-Lebrero, Andrea V Gamarnik, Sergio B Kaufman

Publikováno v: PLoS ONE, Vol 8, Iss 3, p e58508 (2013)

Dengue virus nonstructural protein 3 (NS3) unwinds double stranded RNA driven by the free energy derived from the hydrolysis of nucleoside triphosphates. This paper presents the first systematic and quantitative characterization of the steady-state N

Externí odkaz: https://doaj.org/article/21941bb1968a4174a29a1c9a9c78ee51

Extracellular ATP hydrolysis in Caco-2 human intestinal cell line

Autor: Maria Paula Faillace, Cora Lilia Alvarez, Gerardo Raul Corradi, J. Schachter, Mariano A. Ostuni, M. Pucci Molineris, Z. Bazzi, Claude Hattab, Pablo J. Schwarzbaum, Jean Sévigny, Débora E. Rinaldi, Rodolfo M. González-Lebrero

Publikováno v: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1863:183679

Extracellular nucleotides and nucleosides activate signaling pathways that play major roles in the physiology and pathophysiology of the gastrointestinal tract. Ectonucleotidases hydrolyze extracellular nucleotides and thus regulate ligand exposure t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce846e737ec7d9ded307409740e98221
https://doi.org/10.1016/j.bbamem.2021.183679

Thermodynamic study of the effect of ions on the interaction between dengue virus NS3 helicase and single stranded RNA

Autor: Ernesto A. Roman, Leila Alejandra Cababie, Leopoldo German Gebhard, Rodolfo M. González-Lebrero, Sergio B. Kaufman, Juan Jeremías Incicco, Andrea V. Gamarnik

Publikováno v: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)

Dengue virus nonstructural protein 3 (NS3) fulfills multiple essential functions during the viral replication and constitutes a prominent drug target. NS3 is composed by a superfamily-2 RNA helicase domain joined to a serine protease domain. Quantita

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0efaf3fce32c0ab051eb4a0ded270b0e
https://www.nature.com/articles/s41598-019-46741-4

A laboratory work to introduce biochemistry undergraduate students to basic enzyme kinetics-alkaline phosphatase as a model

Autor: Rodolfo M. González Lebrero, Lorena Gonzalez, Ana I. Sotelo, Johanna G. Miquet

Publikováno v: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET

Enzyme kinetics is an essential topic in undergraduate Biochemistry courses. A laboratory work that covers the principal basic concepts of enzyme kinetics in steady state is presented. The alkaline phosphatase catalyzed reaction of phenyl-phosphate h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e34e127b41aaa46fa0cf0b15dc2426a1
https://pubmed.ncbi.nlm.nih.gov/30576049