Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Rodney S. Roche"'
Publikováno v:
Molecular Microbiology. 12:747-759
Summary Using deletion mutants, It is shown that part of the prosequence, the Ω-peptide (-4, -24), of the thermolysin-like neutral protease (TNP) from Bacillus cereus, Cnp, is not required for efficient processing and secretion of fully functional m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35207e3f84fbccb509197f900b63648c
https://doi.org/10.1111/j.1365-2958.1994.tb01062.x
https://doi.org/10.1111/j.1365-2958.1994.tb01062.x
Publikováno v:
Protein Engineering, Design and Selection.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9bc8d6cbe75dccdd1ef8570dea6bcd14
https://doi.org/10.1093/protein/6.supplement.34
https://doi.org/10.1093/protein/6.supplement.34
Publikováno v:
Molecular microbiology. 6(12)
Summary The Bacillus cereus cnp gene coding for the thermolysin-like neutral protease (TNP) has been cloned, sequenced, and expressed in Bacillus subtilis. The protease is first produced as a pre-pro-protein (Mr= 61000); the pro-peptide is approximat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e33277cc1b363881574edd72efd1e9de
https://pubmed.ncbi.nlm.nih.gov/1495388
https://pubmed.ncbi.nlm.nih.gov/1495388
Publikováno v:
Canadian Journal of Biochemistry. 52:981-990
The physicochemical properties of the extracellular protease of the fungus Malbranchea pulchella, for which we have adopted the name thermomycolase, were investigated. The molecular weight of diisopropylphosphorylthermomycolase was found to be 32 000
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a1301092f01b227bce302cb530b81f2
https://doi.org/10.1139/o74-137
https://doi.org/10.1139/o74-137
Publikováno v:
Analytical Biochemistry. 70:313-326
An equation is found relating the fractional activity, (v/v0), of an enzyme assay mixture to the total concentrations of metalloenzyme, active site metal ion, metal-binding ligand and substrate and the stability constants of the complexes present. Wh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b7db68a005be1ce92f2d19c2c854ea3e
https://doi.org/10.1016/0003-2697(76)90452-8
https://doi.org/10.1016/0003-2697(76)90452-8
Autor:
Rodney S. Roche, Ronald J. T. Corbett
Publikováno v:
Biochemistry. 23:1888-1894
The urea denaturation of sperm whale myoglobin and thermal denaturation of ribonuclease have been studied by following the associated volume changes by size-exclusion chromatography on a Toya Soda TSK 3000SW gel permeation column. The permeation prop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ac99e4b91a3f5191a74daab7af46ac2
https://doi.org/10.1021/bi00303a047
https://doi.org/10.1021/bi00303a047
Publikováno v:
Biochemical and Biophysical Research Communications. 58:8-12
Attention is drawn to the fact that, in comparison to those values derived from sedimentation equilibrium experiments, gel chromatographic methods give anomalously low molecular weights for a variety of proteases. This appears to be particularly true
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d1cc4147a2265de0b639fe36d920f4f
https://doi.org/10.1016/0006-291x(74)90883-3
https://doi.org/10.1016/0006-291x(74)90883-3
Autor:
Gerrit Voordouw, Rodney S. Roche
Publikováno v:
Biochemistry. 13:5017-5021
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d05553f95bc4d91ff3f51ed02144b0c8
https://doi.org/10.1021/bi00721a024
https://doi.org/10.1021/bi00721a024
Publikováno v:
Journal of Analytical and Applied Pyrolysis. 12:19-29
Thermograms of H2S evolved during linearly temperature-programmed pyrolysis of sulphur-containing materials contain peaks corresponding to different modes of H2S generation. Variations in the sulphur isotope composition of the evolved H2S are charact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7f04fd945665e32c11b23f43a38f547c
https://doi.org/10.1016/0165-2370(87)80011-6
https://doi.org/10.1016/0165-2370(87)80011-6
Autor:
Rodney S. Roche, Shlomo Pundak
Publikováno v:
Biochemistry. 23:1549-1555
At physiological pH, bovine testes calmodulin (t-CaM) upon excitation at 278 nm shows typical tyrosine fluorescence at 305 nm and a spectral band characteristic of emission from tyrosinate , at 330-350 nm. In addition, a new band at 312-320 nm appear
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bceda1c29d71c2284437589141deacd2
https://doi.org/10.1021/bi00302a032
https://doi.org/10.1021/bi00302a032