Stable trapping of multiple proteins at physiological conditions using nanoscale chambers with macromolecular gates

Autor: Justas Svirelis, Zeynep Adali, Gustav Emilsson, Jesper Medin, John Andersson, Radhika Vattikunta, Mats Hulander, Julia Järlebark, Krzysztof Kolman, Oliver Olsson, Yusuke Sakiyama, Roderick Y. H. Lim, Andreas Dahlin

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)

Abstract The possibility to detect and analyze single or few biological molecules is very important for understanding interactions and reaction mechanisms. Ideally, the molecules should be confined to a nanoscale volume so that the observation time b

Externí odkaz: https://doaj.org/article/128f0cc5097d4ebdaeac274d69a6839d

Human GBP1 binds LPS to initiate assembly of a caspase-4 activating platform on cytosolic bacteria

Autor: José Carlos Santos, Dave Boucher, Larisa Kapinos Schneider, Benjamin Demarco, Marisa Dilucca, Kateryna Shkarina, Rosalie Heilig, Kaiwen W. Chen, Roderick Y. H. Lim, Petr Broz

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)

Detection of LPS derived from Gram-negative bacteria by innate immune receptors is a critical step in the host response. Here Santos and colleagues show human GBP1 binds to LPS resulting in non-canonical inflammasome activation.

Externí odkaz: https://doaj.org/article/6cea1f719e794133aac06f44406b21f2

Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

Autor: Bita Malekian, Rafael L. Schoch, Timothy Robson, Gustav Ferrand -Drake del Castillo, Kunli Xiong, Gustav Emilsson, Larisa E. Kapinos, Roderick Y. H. Lim, Andreas Dahlin

Publikováno v: Frontiers in Chemistry, Vol 6 (2018)

Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic n

Externí odkaz: https://doaj.org/article/f4395249f0ee41b784b7e4c0be8869f5

Analysis of Tau/Nucleoporin Interactions by Surface Plasmon Resonance Spectroscopy

Autor: Lisa Diez, Larisa E. Kapinos, Roderick Y. H. Lim, Susanne Wegmann

Publikováno v: New York, NY : Springer US, Methods in Molecular Biology 2551, 95-109 (2023). doi:10.1007/978-1-0716-2597-2_8
Protein Aggregation / Cieplak, Andrzej Stanisław (Editor) ; New York, NY : Springer US, 2023, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2596-5=978-1-0716-2597-2 ; doi:10.1007/978-1-0716-2597-2
Methods in Molecular Biology ISBN: 9781071625965

Tau, a soluble and predominantly neuronal protein, is best known for its microtubule (MT)-binding function in the cytosol, where it decisively contributes to stability as well as modulation of MT dynamics. In Alzheimer's disease and other tauopathies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e777ba461e2c7b59cc939f24412a100d
https://pub.dzne.de/record/165566

Rotation characteristics of acoustically actuated thin-film rotors in air and water

Autor: Shichao Jia, Yohei Sato, Roderick Y. H. Lim, Soichiro Tsujino

Publikováno v: 2022 IEEE International Ultrasonics Symposium (IUS).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4e701b87a24c85ce0d5cbd3a3f7a0197
https://doi.org/10.1109/ius54386.2022.9958207