Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Rochelle Aw"'
Publikováno v:
Synthetic and Systems Biotechnology, Vol 5, Iss 3, Pp 137-144 (2020)
A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic gene network design, biosensing and on-demand bio
Externí odkaz:
https://doaj.org/article/2c14f66d13b440f7990cf46e39759377
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Virus-like particles (VLPs) are supramolecular protein assemblies with the potential for unique and exciting applications in synthetic biology and medicine. Despite the attention VLPs have gained thus far, considerable limitations still persist in th
Externí odkaz:
https://doaj.org/article/135577779d2848fda05dce69ca054282
Publikováno v:
Synthetic and Systems Biotechnology, Vol 3, Iss 1, Pp 64-75 (2018)
Adaptation allows organisms to maintain a constant internal environment, which is optimised for growth. The unfolded protein response (UPR) is an example of a feedback loop that maintains endoplasmic reticulum (ER) homeostasis, and is characteristic
Externí odkaz:
https://doaj.org/article/946be861930b44bb94f3bfa50fb457f3
Autor:
Nico Callewaert, Bram Laukens, Karen M. Polizzi, Derek Bell, Rochelle Aw, Riet De Rycke, Michiel De Bruyne, Charlot De Wachter
Publikováno v:
Traffic. 22:48-63
The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes. Here we investigated the link between the structural organi
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2433
Pichia pastoris (syn. Komagataella phaffii) is an industrially relevant recombinant protein platform that has been used to produce over 5000 proteins to date. Cell-free protein synthesis can be used as a screening tool before strain development or fo
Publikováno v:
Cell-Free Gene Expression ISBN: 9781071619971
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a2a39c442ade95e4da833d98b597b69
https://doi.org/10.1007/978-1-0716-1998-8_4
https://doi.org/10.1007/978-1-0716-1998-8_4
Publikováno v:
Methods in enzymology. 660
The methylotrophic yeast Pichia pastoris is currently one of the most versatile and popular hosts for the production of heterologous proteins, including industrial enzymes. The popularity of P. pastoris stems from its ability to grow to high cell den
Autor:
Bryn Edwards-Jones, Rochelle Aw, Geraint R Barton, Gregory D Tredwell, Jacob G Bundy, David J Leak
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0119637 (2015)
We have followed a typical fed-batch induction regime for heterologous protein production under the control of the AOX1 promoter using both microarray and metabolomic analysis. The genetic constructs involved 1 and 3 copies of the TRY1 gene, encoding
Externí odkaz:
https://doaj.org/article/42b7faedf3fb421182eb7583d5545120
Publikováno v:
Methods in Enzymology ISBN: 9780323907378
The methylotrophic yeast Pichia pastoris is currently one of the most versatile and popular hosts for the production of heterologous proteins, including industrial enzymes. The popularity of P. pastoris stems from its ability to grow to high cell den
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6a762f6bc3b3c22046cdd6db6c1f6bf7
https://doi.org/10.1016/bs.mie.2021.07.004
https://doi.org/10.1016/bs.mie.2021.07.004
Publikováno v:
Current Protocols in Protein Science. 102
Cell-free protein synthesis is a powerful tool for engineering biology and has been utilized in many diverse applications, from biosensing and protein prototyping to biomanufacturing and the design of metabolic pathways. By exploiting host cellular m