Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Autor: Christopher A. Ahern, Harley T. Kurata, Stephan A. Pless, Robin Y. Kim

Publikováno v: The Journal of Physiology. 593:2627-2634

Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4e24d0ff3e8960896e47654f116f60ec
https://doi.org/10.1113/jphysiol.2014.287714

PIP2 mediates functional coupling and pharmacology of neuronal KCNQ channels

Autor: Robin Y. Kim, Stephan A. Pless, Harley T. Kurata

Publikováno v: Kim, R Y, Pless, S A & Kurata, H T 2017, ' PIP2 mediates functional coupling and pharmacology of neuronal KCNQ channels ', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 45, pp. E9702–E9711 . https://doi.org/10.1073/pnas.1705802114
Proceedings of the National Academy of Sciences of the United States of America

Significance Despite the availability of many drugs to treat epilepsy, nearly one-third of patients are not responsive to pharmacotherapy. Retigabine (RTG) is the first approved antiepileptic drug that acts by promoting activation of potassium channe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0814b3633050918cf577226df6ce3389
https://doi.org/10.1073/pnas.1705802114

Decomposition of Slide Helix Contributions to ATP-dependent Inhibition of Kir6.2 Channels

Autor: Runying Yang, Roger S. Zhang, Robin Y. Kim, Xinyang Huang, Harley T. Kurata, Jenny B.W. Li

Publikováno v: Journal of Biological Chemistry. 288:23038-23049

Regulation of inwardly rectifying potassium channels by intracellular ligands couples cell membrane excitability to important signaling cascades and metabolic pathways. We investigated the molecular mechanisms that link ligand binding to the channel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ad4a6e3163ecaeec5884b1d4793a903
https://doi.org/10.1074/jbc.m113.485789

Atomic basis for therapeutic activation of neuronal potassium channels

Autor: Christopher A. Ahern, Guiscard Seebohm, Michael C. Yau, Jason D. Galpin, Stephan A. Pless, Robin Y. Kim, Harley T. Kurata

Publikováno v: Nature Communications

Retigabine is a recently approved anticonvulsant that acts by potentiating neuronal M-current generated by KCNQ2–5 channels, interacting with a conserved Trp residue in the channel pore domain. Using unnatural amino-acid mutagenesis, we subtly alte

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d68f3e2e81234868ad274e1fba344876
http://europepmc.org/articles/PMC4561856

Use-dependent activation of neuronal Kv1.2 channel complexes

Autor: Victoria A. Baronas, Brandon R. McGuinness, G. Stefano Brigidi, Rachel N. Gomm Kolisko, Yury Y. Vilin, Robin Y. Kim, Francis C. Lynn, Shernaz X. Bamji, Runying Yang, Harley T. Kurata

Publikováno v: The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(8)

In excitable cells, ion channels are frequently challenged by repetitive stimuli, and their responses shape cellular behavior by regulating the duration and termination of bursts of action potentials. We have investigated the behavior ofShakerfamily

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5633935cc94742a48b3c726649136167
https://pubmed.ncbi.nlm.nih.gov/25716850