Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Robin, Hurst"'
Autor:
Emily A. Torio, Valerie T. Ressler, Virginia A. Kincaid, Robin Hurst, Mary P. Hall, Lance P. Encell, Kristopher Zimmerman, Stuart K. Forsyth, William M. Rehrauer, Molly A. Accola, Chia-Chang Hsu, Thomas Machleidt, Melanie L. Dart
Publikováno v:
Frontiers in Microbiology, Vol 13 (2022)
Point-of-care tests are highly valuable in providing fast results for medical decisions for greater flexibility in patient care. Many diagnostic tests, such as ELISAs, that are commonly used within clinical laboratory settings require trained technic
Externí odkaz:
https://doaj.org/article/9343fd9d3e9b4dcaa378e95d0d22ecaf
Publikováno v:
Bio-Protocol, Vol 10, Iss 24 (2020)
G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeuti
Externí odkaz:
https://doaj.org/article/30c0400fe0cb45b6ab6b4948fd1821d6
Autor:
Michael P. Killoran, Sergiy Levin, Michelle E. Boursier, Kristopher Zimmerman, Robin Hurst, Mary P. Hall, Thomas Machleidt, Thomas A. Kirkland, Rachel Friedman Ohana
Publikováno v:
Molecules, Vol 26, Iss 10, p 2857 (2021)
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research. NanoLuc-based bioluminescence resonance energy transfer (Na
Externí odkaz:
https://doaj.org/article/6603ab2a2c3e4fc183fbdeba047372cc
Autor:
Stuart K Forsyth, Emily Jost, Monika G. Wood, Valerie T Ressler, Dan Lazar, Kris Zimmermann, Connor Fitzgerald, Thomas A. Kirkland, Mary P. Hall, Thomas Machleidt, Thomas P. Smith, Kincaid Virginia, Keith V. Wood, Robin Hurst, Lance P. Encell, Melanie Dart
Publikováno v:
Analytical Chemistry. 93:5177-5184
Enzyme-linked immunosorbent assays (ELISAs) are used extensively for the detection and quantification of biomolecules in clinical diagnostics as well as in basic research. Although broadly used, the inherent complexities of ELISAs preclude their util
Autor:
Rachel Friedman Ohana, Keith V. Wood, Robin Hurst, Kristopher Zimmerman, Michael M. Rosenblatt, Sergiy Levin, Thomas Machleidt, Thomas A. Kirkland
Publikováno v:
ACS Chemical Biology. 16:404-413
Identification of physiologically relevant targets for lead compounds emerging from drug discovery screens is often the rate-limiting step toward understanding their mechanism of action and potential for undesired off-target effects. To this end, we
Autor:
Mary P. Hall, Mark A. Kay, Yunhee Park, Joel R. Walker, Lan Xiang Liu, Louai Labanieh, Robbie G. Majzner, Namdoo Kim, Michael Z. Lin, Lance P. Encell, Jennifer R. Cochran, Kerriann M. Casey, David C. Wang, Robin Hurst, Yichi Su, Thomas A. Kirkland, Feijie Zhang, Crystal L. Mackall, Thomas P. Smith
Publikováno v:
Nature Methods. 17:852-860
Sensitive detection of two biological events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal
Autor:
Michelle E. Boursier, Thomas Machleidt, Christopher T. Eggers, Keith V. Wood, Robin Hurst, Kris Zimmerman, Thomas A. Kirkland, Braeden L. Butler, Rachel Friedman Ohana, Sergiy Levin
Publikováno v:
J Biol Chem
G protein-coupled receptors (GPCRs) are prominent targets to new therapeutics for a range of diseases. Comprehensive assessments of their cellular interactions with bioactive compounds, particularly in a kinetic format, are imperative to the developm
Autor:
Matthew B. Robers, James D. Vasta, Cesear R. Corona, Rachel Friedman Ohana, Robin Hurst, Manisha A. Jhala, Kenneth M. Comess, Keith V. Wood
Intracellular target affinity and residence time are fundamental aspects of pharmacological mechanism (Lu and Tonge, Curr Opin Chem Biol 14:467–474, 2010). Although various robust biochemical approaches exist to measure these binding characteristic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4252113f5cb0427bf28cb64d7e2d3a93
https://doi.org/10.17504/protocols.io.bqpmmvk6
https://doi.org/10.17504/protocols.io.bqpmmvk6
Autor:
Sergiy Levin, Keith V. Wood, Rachel Friedman Ohana, Robin Hurst, Matthew B. Robers, Thomas A. Kirkland, Thomas Machleidt, Mike Rosenblatt, Lance P. Encell
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
Ligand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous pop
Highly Efficient Protein and Complex Purification from Mammalian Cells Using the HaloTag® Technology
Autor:
Jacqui L. Méndez, Rachel Friedman Ohana, Robin Hurst, Nancy Murphy, Hélène Benink, Michael R. Slater, Chad Brueck, Danette L. Daniels, Marjeta Urh
Publikováno v:
BioTechniques, Vol 51, Iss 4, Pp 276-277 (2011)
Externí odkaz:
https://doaj.org/article/799f7b0d8f414edda4e13038f7a46a97