Výsledky vyhledávání - "Roberto Munilla"

Intense PEGylation of Enzyme Surfaces

Autor: Jose M. Guisan, Alejandro H. Orrego, Sonia Moreno-Pérez, Roberto Munilla, S. Martins-DeOliveira, Maria Romero-Fernández, Gloria Fernández-Lorente, Lara Trobo-Maseda

This chapter describes the physicochemical coating of the surface of immobilized enzymes with a dense layer of polyethylene glycol (PEG) to improve enzyme stability. One hypothesis is that a dense, viscous, polar PEG layer around the enzyme would enh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5f345bde6acfb912561531b1d4a7091d
https://doi.org/10.1016/bs.mie.2016.02.016

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Glutaraldehyde Cross-Linking of Lipases Adsorbed on Aminated Supports in the Presence of Detergents Leads to Improved Performance

Autor: Jose M. Palomo, Claudia Ortiz, Zaida Cabrera, Cesar Mateo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, Jose M. Guisan, Roberto Munilla

Publikováno v: Biomacromolecules. 7:2610-2615

Lipases from Candida rugosa (CRL) and lipase isoforms A and B from Candida antarctica (CAL-A and CAL-B) were adsorbed on aminated supports in the presence of detergents to have individual lipase molecules. Then, one fraction was washed to eliminate t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a668b997e9c8443de01a5d1667627935
https://doi.org/10.1021/bm060408+

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Supports coated with PEI as a new tool in chromatography

Autor: Roberto Munilla, Manuel Fuentes, Roberto Fernandez-Lafuente, Benevides C. C. Pessela, Rodrigo Torres, Cesar Mateo, Jose M. Guisan

Publikováno v: Enzyme and Microbial Technology. 39:711-716

PEI coated supports are proposed in this manuscript as a matrix that may permit a new concept in chromatography. Standard supports have their active groups on a “plane” surface, and permit the interaction with an area accounting for not more than

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3076c06c11be9a1cada25157f7828548
https://doi.org/10.1016/j.enzmictec.2005.12.006

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Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions

Autor: Manuel Fuentes, Alfonso V. Carrascosa, Roberto Munilla, Jose M. Guisan, Cesar Mateo, Roberto Fernandez-Lafuente, Benevides C. C. Pessela

Publikováno v: Enzyme and Microbial Technology. 39:909-915

The interaction of two large beta-galactosidases (from Escherichia coli and from Thermus sp.) with tailor-made anion exchangers was studied. Using lowly activated supports (e.g., containing 2–3 μmol of ionised groups per wet gram of support), larg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e5a2a17d6f6be712d2ef395e7eb4b6dc
https://doi.org/10.1016/j.enzmictec.2006.01.024

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Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices

Autor: Alejandro Vian, Alfonso V. Carrascosa, Benevides C. C. Pessela, José Luis García, Jose M. Guisan, Rodrigo Torres, Roberto Munilla, Roberto Fernandez-Lafuente, Manuel Fuentes, Cesar Mateo

Publikováno v: Journal of Chromatography A. 1055:93-98

A strategy to selectively adsorb large proteins on immobilized metal ion affinity chromatography supports is presented. It is based on the fact that large proteins have a large surface that permits the long distance interaction with groups placed qui

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::351201c53679f26cfc5cbe12f301d0bc
https://doi.org/10.1016/j.chroma.2004.08.141

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Stabilization of enzymes by multipoint attachment via reversible immobilization on phenylboronic activated supports

Autor: Manuel Fuentes, Cesar Mateo, Roberto Fernandez-Lafuente, Jose M. Guisan, Benevides C. C. Pessela, Rodrigo Torres, Roberto Munilla

Publikováno v: Journal of biotechnology. 120(4)

In this work, we have used supports activated with m-amino-phenylboronic groups to "reversibly" immobilize proteins under very mild conditions. Most of the proteins contained in a crude extract from E. coli could be immobilized on Eupergit C-250 L ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aff58190c68469507e531a4116c47b4
https://pubmed.ncbi.nlm.nih.gov/16129505

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Detection and purification of two antibody-antigen complexes via selective adsorption on lowly activated anion exchangers

Autor: Manuel Fuentes, Jose M. Guisan, Cesar Mateo, Roberto Munilla, Roberto Fernandez-Lafuente, Benevides C. C. Pessela

Publikováno v: Journal of chromatography. A. 1059(1-2)

Taken advantage of the mechanism of adsorption of macro-molecules on ionic exchangers, (a multipoint interaction between the protein and the support), it is possible to selectively adsorb large proteins leaving small ones in the supernatant. Associat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7784b12bc02fb4add3fe527ea2b696c7
https://pubmed.ncbi.nlm.nih.gov/15628128

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Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices. Purification of multimeric thermophilic enzymes overexpressed in Escherichia coli

Autor: Benevides C C, Pessela, Rodrigo, Torres, Manuel, Fuentes, Cesar, Mateo, Roberto, Munilla, Alejandro, Vian, Alfonso V, Carrascosa, Jose L c, Garcia, Jose M, Guisán, Roberto, Fernandez-Lafuente

Publikováno v: Journal of chromatography. A. 1055(1-2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::59cf4f3d9b4488a75f8974435d35da4c
https://pubmed.ncbi.nlm.nih.gov/15560484

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Ion exchange using poorly activated supports, an easy way for purification of large proteins

Autor: Jose M. Guisan, Alejandro Vian, Roberto Fernandez-Lafuente, Manuel Fuentes, Alfonso V. Carrascosa, Roberto Munilla, Benevides C. C. Pessela, Lorena Betancor

Publikováno v: Journal of chromatography. A. 1034(1-2)

Ion-exchange chromatography using commercial ionic supports is a commonly used technique for protein purification. However, selective adsorption of a target protein from a given extract onto commercial ion exchangers seems to be quite complex since t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::901a22752ce556d5bc500ff3c8340705
https://pubmed.ncbi.nlm.nih.gov/15116925

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