Modeling Hippocampal Neurogenesis Using Human Pluripotent Stem Cells

Autor: Diana Xuan Yu, Francesco Paolo Di Giorgio, Jun Yao, Maria Carolina Marchetto, Kristen Brennand, Rebecca Wright, Arianna Mei, Lauren Mchenry, David Lisuk, Jaeson Michael Grasmick, Pedro Silberman, Giovanna Silberman, Roberto Jappelli, Fred H. Gage

Publikováno v: Stem Cell Reports, Vol 2, Iss 3, Pp 295-310 (2014)

The availability of human pluripotent stem cells (hPSCs) offers the opportunity to generate lineage-specific cells to investigate mechanisms of human diseases specific to brain regions. Here, we report a differentiation paradigm for hPSCs that enrich

Externí odkaz: https://doaj.org/article/5026ef6663eb4d668d41b5443ec01167

Short promoters in viral vectors drive selective expression in mammalian inhibitory neurons, but do not restrict activity to specific inhibitory cell-types

Autor: Jason L Nathanson, Roberto Jappelli, Eric D Scheeff, Gerard Manning, Kunihiko Obata, Sydney Brenner, Edward M Callaway

Publikováno v: Frontiers in Neural Circuits, Vol 3 (2009)

Short cell-type specific promoter sequences are important for targeted gene therapy and studies of brain circuitry. We report on the ability of short promoter sequences to drive fluorescent protein expression in specific types of mammalian cortical i

Externí odkaz: https://doaj.org/article/d51eb07e87f34bc8a1b1eaee02b2f2e4

Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology

Autor: Fred H. Gage, A K Bryant, Bart P. F. Rutten, Roger S. Lasken, Mark A.J. Gorris, Roberto Jappelli, Jennifer A. Erwin, A A Paucar, Gene W. Yeo, Ruben V. Hernandez, M van den Hurk, Jerika J. Barron, Baptiste N. Jaeger, Mariko Kellogg, Cynthia Marchand, Boyko Kakaradov, Harry W.M. Steinbusch, Tameji Eames, Cedric Bardy

Publikováno v: Molecular Psychiatry, 21, 1573-1588
Molecular Psychiatry, 21, 11, pp. 1573-1588
Molecular Psychiatry, 21(11), 1573-1588. Nature Publishing Group

Item does not contain fulltext Human neural progenitors derived from pluripotent stem cells develop into electrophysiologically active neurons at heterogeneous rates, which can confound disease-relevant discoveries in neurology and psychiatry. By com

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c64042e85ac15f84e673d706b3213bc
https://pubmed.ncbi.nlm.nih.gov/27698428

Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro

Autor: Arianna Mei, Maria C. Marchetto, Cedric Bardy, Krishnan Padmanabhan, Stacy Kim, David H. Adamowicz, Apuã C. M. Paquola, Matthew Shtrahman, Jennifer A. Erwin, Xin Jin, Hyung J. Kim, Jason R. Klug, Roberto Jappelli, Manching Ku, Fred H. Gage, Anindita Sarkar, Shani Stern, Neda Neshat, Maxim N. Shokhirev

Publikováno v: Cell Stem Cell. 22:684-697.e9

Despite widespread interest in using human induced pluripotent stem cells (hiPSCs) in neurological disease modeling, a suitable model system to study human neuronal connectivity is lacking. Here, we report a comprehensive and efficient differentiatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26f50304b6927cb3903135835631d83b
https://doi.org/10.1016/j.stem.2018.04.009

A Genetic Screen to Identify Sequences That Mediate Protein Oligomerization in Escherichia coli

Autor: Roberto Jappelli, Sydney Brenner

Publikováno v: Biochemical and Biophysical Research Communications. 266:243-247

Many proteins assemble as oligomeric complexes and in several cases a distinct domain mediates the interaction between the subunits. The identification of new oligomerization modules is relevant to comprehend both the architecture and the evolution o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d992f2c895ba8874e7cf442d2745757
https://doi.org/10.1006/bbrc.1999.1806