Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes

Autor: Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera, Lawrence J. Parkhurst

Publikováno v: Biosensors, Vol 10, Iss 11, p 180 (2020)

The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), po

Externí odkaz: https://doaj.org/article/7dfaeab36ec547de87ec5e260492acf3

A Platform analytical method for intact polysorbates in protein-containing biopharmaceutical products via HPLC-CAD

Autor: Katie A. Carnes, Lee D. Oliver, Timothy A. Brown, Roberto F. Delgadillo, Michelle S. Ward, Sina Mortazavi, Michael J. Morris, Justin W. Shearer, Joshua S. Fuller

Polysorbate (also known as “Tween”) 80 (PS80) is the most common surfactant in biopharmaceuticals. Here, we report a sensitive, and specific platform analytical method using reversed-phase high performance liquid chromatography employing a charge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e43ebec61433c41a7c8d8378a5ebe04

Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin

Autor: Timothy C. Mueser, Katie A. Carnes, Lawrence J. Parkhurst, Kathia Zaleta-Rivera, José González-Valdez, Roberto F. Delgadillo

Publikováno v: PLoS ONE, Vol 14, Iss 2, p e0204194 (2019)
PLoS ONE

The high affinity (KD ~ 10−15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73039636a968c86dff7b47ff33d34af0
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce

Computational and biophysical approaches to protein–protein interaction inhibition of Plasmodium falciparum AMA1/RON2 complex

Autor: Roberto F. Delgadillo, Maryse Lebrun, Martine Pugnière, Michelle L. Tonkin, Dominique Douguet, Martin J. Boulanger, Emilie Pihan

Publikováno v: Journal of Computer-Aided Molecular Design
Journal of Computer-Aided Molecular Design, Springer Verlag, 2015, 29 (6), pp.525-539. ⟨10.1007/s10822-015-9842-7⟩

Invasion of the red blood cell by Plasmodium falciparum parasites requires formation of an electron dense circumferential ring called the Moving Junction (MJ). The MJ is anchored by a high affinity complex of two parasite proteins: Apical Membrane An

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26c2aafeaea45318a9d8482e60cc4168
https://doi.org/10.1007/s10822-015-9842-7

Spectroscopic Properties of Fluorescein and Rhodamine Dyes Attached to DNA

Autor: Roberto F. Delgadillo, Lawrence J. Parkhurst

Publikováno v: Photochemistry and Photobiology. 86:261-272

We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::459c82ab27c84ae9c17cffb9c6f36f6e
https://doi.org/10.1111/j.1751-1097.2009.00663.x