Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Roberto Balbontín"'
Autor:
Julia Aguilera-Herce, Concepción Panadero-Medianero, María Antonia Sánchez-Romero, Roberto Balbontín, Joaquín Bernal-Bayard, Francisco Ramos-Morales
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 9, p 8403 (2023)
Type III secretion systems are found in many Gram-negative pathogens and symbionts of animals and plants. Salmonella enterica has two type III secretion systems associated with virulence, one involved in the invasion of host cells and another involve
Externí odkaz:
https://doaj.org/article/0090b9b773774967b8c8bf4d53086ef5
Publikováno v:
PLoS Biology, Vol 15, Iss 4, p e2001741 (2017)
Mutations conferring resistance to antibiotics are typically costly in the absence of the drug, but bacteria can reduce this cost by acquiring compensatory mutations. Thus, the rate of acquisition of compensatory mutations and their effects are key f
Externí odkaz:
https://doaj.org/article/6366d860f7f24e968aec57303717415b
Publikováno v:
Cold Spring Harbor protocols. 2022(10)
Genomic engineering of Escherichia coli and Salmonella often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0461a062986c5933bcfac2ce8e54c9f
https://pubmed.ncbi.nlm.nih.gov/35960620
https://pubmed.ncbi.nlm.nih.gov/35960620
Publikováno v:
Cold Spring Harbor protocols. 2022(10)
Transduction experiments in Escherichia coli and Salmonella are usually performed with virulent phage variants. A widely used P1 mutant, called P1 vir, carries one or more uncharacterized mutations that prevent formation of lysogens. In the case of P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b12d561889cedac443b83561be646fef
https://pubmed.ncbi.nlm.nih.gov/35960630
https://pubmed.ncbi.nlm.nih.gov/35960630
Publikováno v:
Cold Spring Harbor protocols. 2022(10)
In experimental bacteriology, bacteria are generally manipulated, stored, and shipped in the form of cultures. Depending on various factors, including strain genotype, storage and shipping methods, and manipulator skills, the culture may contain gene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c8856f53503803e621b73ceb2b42cf9
https://pubmed.ncbi.nlm.nih.gov/35960621
https://pubmed.ncbi.nlm.nih.gov/35960621
Publikováno v:
Cold Spring Harbor protocols. 2022(10)
Methods for the in vivo manipulation of bacterial genomes have improved greatly in recent years because of the discovery of new mechanisms and the gigantic leap forward in DNA-sequencing technology. Many cutting-edge approaches still rely on a variet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df5970a86723c337bb5ad5ca84c45cf7
https://pubmed.ncbi.nlm.nih.gov/35960618
https://pubmed.ncbi.nlm.nih.gov/35960618
Publikováno v:
Cold Spring Harbor Protocols. 2022:pdb.prot107853
We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b15403a9390652d1a40d9888fd108e56
https://doi.org/10.1101/pdb.prot107853
https://doi.org/10.1101/pdb.prot107853
Publikováno v:
Cold Spring Harbor Protocols. 2022:pdb.prot107852
The most common method for isolating plasmid DNA is derived from an alkaline lysis procedure. The procedure exploits the differential partitioning of plasmid and chromosomal DNA when denatured by alkali and subsequently renatured by neutralization of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71c2d9f828458df32f44b65d27bac686
https://doi.org/10.1101/pdb.prot107852
https://doi.org/10.1101/pdb.prot107852
Publikováno v:
Molecular Biology and Evolution
Antibiotic resistance often generates defects in bacterial growth called fitness cost. Understanding the causes of this cost is of paramount importance, as it is one of the main determinants of the prevalence of resistances upon reducing antibiotics
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e4fe0114f77cfef1110f682fc5aa070
Publikováno v:
Trends in Microbiology
Antibiotics target essential cellular functions but bacteria can become resistant by acquiring either exogenous resistance genes or chromosomal mutations. Resistance mutations typically occur in genes encoding essential functions; these mutations are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0371d7b31123a583f3b1dec15decd5d
https://doi.org/10.1016/j.tim.2018.01.005
https://doi.org/10.1016/j.tim.2018.01.005