Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Roberto A Chica"'
Publikováno v:
PLoS ONE, Vol 7, Iss 12, p e52463 (2012)
Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little o
Externí odkaz:
https://doaj.org/article/ee29b0349282447eabd95c4725e1af36
Autor:
Andrew C Hemmert, Tamara C Otto, Roberto A Chica, Monika Wierdl, Jonathan S Edwards, Steven M Lewis, Carol C Edwards, Lyudmila Tsurkan, C Linn Cadieux, Shane A Kasten, John R Cashman, Stephen L Mayo, Philip M Potter, Douglas M Cerasoli, Matthew R Redinbo
Publikováno v:
PLoS ONE, Vol 6, Iss 3, p e17441 (2011)
Organophosphorus (OP) nerve agents are potent suicide inhibitors of the essential neurotransmitter-regulating enzyme acetylcholinesterase. Due to their acute toxicity, there is significant interest in developing effective countermeasures to OP poison
Externí odkaz:
https://doaj.org/article/cc3673771a9c4824bb65ae97a1dd1a81
Autor:
Antony D. St-Jacques, Joshua M. Rodriguez, Matthew G. Eason, Scott M. Foster, Safwat T. Khan, Adam M. Damry, Natalie K. Goto, Michael C. Thompson, Roberto A. Chica
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract Structural plasticity of enzymes dictates their function. Yet, our ability to rationally remodel enzyme conformational landscapes to tailor catalytic properties remains limited. Here, we report a computational procedure for tuning conformati
Externí odkaz:
https://doaj.org/article/c5dbd4d40ae840a6a59e282e97bd6c6b
Autor:
Mykhaylo Slobodyanyuk, Jesús A. Banda-Vázquez, Mackenzie J. Thompson, Rebecca A. Dean, John E. Baenziger, Roberto A. Chica, Corrie J. B. daCosta
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-11 (2022)
A structural and functional study of the prokaryotic ligand-gated ion channel, ELIC, provides insight into the origin of agonism and antagonism at nicotinic acetylcholine receptors.
Externí odkaz:
https://doaj.org/article/a19626476cd2418fa92c83a006ffee5b
Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico
Autor:
Aron Broom, Rojo V. Rakotoharisoa, Michael C. Thompson, Niayesh Zarifi, Erin Nguyen, Nurzhan Mukhametzhanov, Lin Liu, James S. Fraser, Roberto A. Chica
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Kemp eliminases are artificial enzymes that catalyze the concerted deprotonation and ring-opening of benzisoxazoles. Here, the authors use room-temperature X-ray crystallography to investigate changes to the conformational ensemble of the Kemp elimin
Externí odkaz:
https://doaj.org/article/7439f4de0efc41d198a2176b909a8ff0
Autor:
Sandrine Legault, Derek P. Fraser-Halberg, Ralph L. McAnelly, Matthew G. Eason, Michael C. Thompson, Roberto A. Chica
Publikováno v:
Chemical Science. 13:1408-1418
We used computational design to increase quantum yield in a fluorescent protein by optimizing chromophore packing to reduce non-radiative decay, resulting in an >10-fold increase in quantum yield that was further improved by directed evolution.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e4d2ff68ca6c28d837740154ad7bf9c1
https://doi.org/10.1039/d1sc05088e
https://doi.org/10.1039/d1sc05088e
Autor:
Roberto A Chica, Brett M Garabedian
Publikováno v:
Protein Engineering, Design and Selection. 36
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4537ecb9d2223533fe383af141c6da37
https://doi.org/10.1093/protein/gzad004
https://doi.org/10.1093/protein/gzad004
Autor:
Antony D. St-Jacques, Joshua M. Rodriguez, Matthew G. Eason, Scott M. Foster, Safwat T. Khan, Adam M. Damry, Natalie K. Goto, Michael C. Thompson, Roberto A. Chica
Structural plasticity of enzymes dictates their function. Yet, our ability to rationally remodel enzyme conformational landscapes to tailor catalytic properties remains limited. Here, we report a computational procedure for tuning conformational land
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bc851cb51fb15e02aeab27097d7f72cf
https://doi.org/10.1101/2022.09.16.508321
https://doi.org/10.1101/2022.09.16.508321
Autor:
Michael Ong, Bryan Lo, Marie-Ève Wedge, Carolina S. Ilkow, John C. Bell, Catia Cemeus, Oliver Varette, Aron Broom, Benjamin McSweeney, Bruce F. Burns, Harold L. Atkins, Brian J Laight, Julia Petryk, Brian A. Keller, Jean-Simon Diallo, Carolyn Nessim, Roberto A. Chica
Publikováno v:
Journal of Cancer Research and Clinical Oncology. 147:1365-1378
Mutations in BRAF are the most prominent activating mutations in melanoma and are increasingly recognized in other cancers. There is currently no accepted treatment regimen for patients with mutant BRAFK601N melanoma, and the study of melanoma driven
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6ae1f24727701df369e59dd2c32a58f0
https://doi.org/10.1007/s00432-021-03545-2
https://doi.org/10.1007/s00432-021-03545-2
Autor:
Sandrine, Legault, Derek P, Fraser-Halberg, Ralph L, McAnelly, Matthew G, Eason, Michael C, Thompson, Roberto A, Chica
Publikováno v:
Chemical science. 13(5)
Red fluorescent proteins (RFPs) have found widespread application in chemical and biological research due to their longer emission wavelengths. Here, we use computational protein design to increase the quantum yield and thereby brightness of a dim mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f96c9abb09e43dbe73a38b9e5269ad99
https://pubmed.ncbi.nlm.nih.gov/35222925
https://pubmed.ncbi.nlm.nih.gov/35222925