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of 10
pro vyhledávání: '"Roberta N. Garcia"'
Autor:
Rona Karmela D. Bravo, Mark Rickard N. Angelia, Lawrence Yves C. Uy, Roberta N. Garcia, Mary Ann O. Torio
Publikováno v:
Food Chemistry: Molecular Sciences, Vol 4, Iss , Pp 100062- (2022)
Previous studies in pigeon pea showed health benefits but very few explored peptide bioactivities. This study examined antimicrobial, antioxidant, and antihypertensive activities of peptides in purified and proteolyzed major globulin fraction, cajani
Externí odkaz:
https://doaj.org/article/9f46f6092873407d80c0aae81f52fd36
Publikováno v:
International Journal of Philippine Science and Technology. 8:46-51
Cocosin, the 11S globulin of coconut, is the major storage protein that accumulates in the endosperm during seed development. This work describes the gene structure, derived amino acid sequence, structural homologies, and developmental expression pro
Publikováno v:
Journal of Biological Sciences. 12:393-399
Autor:
Nobuyuki Maruyama, Evelyn Mae Tecson-Mendoza, Mary Ann O. Torio, Shigeru Utsumi, Takafumi Itoh, Roberta N. Garcia
Publikováno v:
Food Research International. 45:277-282
Sulfhydryl groups and disulfide bond were introduced in mungbean's major storage protein, 8Sα globulin, by protein engineering to improve structural stability and functional properties. Five modified proteins or mutants (F59C, I99C, A213C, one free
Autor:
Shigeru Utsumi, Nobuyuki Maruyama, Krisna Prak, Motoyasu Adachi, Evelyn Mae Tecson-Mendoza, Mary Ann O. Torio, Roberta N. Garcia
Publikováno v:
Food Research International. 44:2984-2990
The major storage protein of mungbean, 8Sα globulin or vicilin, was engineered using site-directed mutagenesis to increase the number of methionine (Met) residues in the molecule for improvement of functional and nutritional qualities. Eight Met-ric
Autor:
Roberta N. Garcia, Kristian Mark P. Caldo, Shigeru Utsumi, Evelyn Mae Tecson-Mendoza, Mark Rickard N.Angelia, Krisna Prak
Publikováno v:
Food Science and Technology Research. 16:225-232
The physicochemical and functional characteristics of the major coconut storage protein, 11S globulin or cocosin, were investigated.Cocosin was purified by a combination of salt extraction, selective precipitation, and gel filtration chromatography.
Autor:
Shigeru Utsumi, and Amy Emiliana N. Bernardo, Roberta N. Garcia, Motoyasu Adachi, Evelyn Mae Tecson-Mendoza
Publikováno v:
Journal of Agricultural and Food Chemistry. 54:6005-6010
We have previously cloned and characterized the cDNAs of three isoforms of the 8S globulin of mungbean, expressed the major 8Salpha isoform in Escherichia coli, and purified and successfully crystallized it (Bernardo, A. E. N.; Garcia, R. N.; Adachi,
Autor:
Bunzo Mikami, Roberta N. Garcia, Motoyasu Adachi, Shigeru Utsumi, Yukie Maruyama, Evelyn Mae Tecson-Mendoza, Takafumi Itoh
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 62(Pt 7)
The 8S globulins of mung bean [Vigna radiata (L.) Wilczek] are vicilin-type seed storage globulins which consist of three isoforms: 8Salpha, 8Salpha' and 8Sbeta. The three isoforms have high sequence identities with each other (around 90%). The struc
Publikováno v:
Journal of agricultural and food chemistry. 53(5)
Total globulins extracted with 0.4 M NaCl in buffer from coconut endosperm separated into two peaks on gel filtration: peak I corresponding to 11S globulin or cocosin and peak II to 7S globulin with native molecular weights of 326 000 and 156 000, re
Autor:
Roberta N. Garcia, Shigeru Utsumi, Evelyn Mae Tecson-Mendoza, Amy Emiliana N. Bernardo, Akito Kaga, Motoyasu Adachi, Jorge Gil C. Angeles, Ishimoto Masao
Publikováno v:
Journal of agricultural and food chemistry. 52(9)
Three isoforms of the cDNA of the major 8S globulin of mungbean, 8Salpha, 8Salpha', and 8Sbeta, were isolated, cloned, and characterized. The cDNA sequences of 8Salpha, 8Salpha', and 8Sbeta had open reading frames of 1362, 1359 or 1362, and 1359 bp,