Detection of high-risk HPV in FFPE specimens of various tumours using the BD Onclarity™ HPV Assay

Autor: Robert van der Geize, Natalie Methorst, Maarten Niemantsverdriet

Publikováno v: Tumour Virus Research, Vol 14, Iss , Pp 200243- (2022)

Analysis of high-risk HPV status on formalin-fixed paraffin-embedded (FFPE) tissue material is valuable for cervical-, head and neck-, anogenital- and other types of cancer, but commercial HPV assays have been developed specifically for cervix swab c

Externí odkaz: https://doaj.org/article/d767b202bc0446db97c52ddd2a202671

3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1 Contains a Phosphatidylinositol Cofactor

Autor: Stefania Montersino, Evelien te Poele, Roberto Orru, Adrie H. Westphal, Arjan Barendregt, Albert J. R. Heck, Robert van der Geize, Lubbert Dijkhuizen, Andrea Mattevi, Willem J. H. van Berkel

Publikováno v: Frontiers in Microbiology, Vol 8 (2017)

3-Hydroxybenzoate 6-hydroxylase (3HB6H, EC 1.13.14.26) is a FAD-dependent monooxygenase involved in the catabolism of aromatic compounds in soil microorganisms. 3HB6H is unique among flavoprotein hydroxylases in that it harbors a phospholipid ligand.

Externí odkaz: https://doaj.org/article/9126747169364aec8fb7bd8a50f81ac5

Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing

Autor: Joost Vermaat, Tom van Wezel, Paula J P de Vree, Wouter de Laat, Bauke Ylstra, Amin Allahyar, Marieke Simonis, Harma Feitsma, Adrien S. J. Melquiond, Max van Min, Agata Rakszewska, Erik Splinter, Daphne de Jong, Joost Swennenhuis, Milan Sharma, Mehmet Yilmaz, Arjan Diepstra, Roos J Leguit, Robert van der Geize, Phylicia Stathi, Karima Hajo, Nathalie J. Hijmering, Mark Pieterse, Marjon J.A.M. Verstegen, Peter H.L. Krijger, Ruud W J Meijers, G Tjitske Los-de Vries, Léon C van Kempen, Arjen H.G. Cleven

Publikováno v: Nature Communications, 12(1):3361. Nature Publishing Group
Nature Communications
Nature Communications, 12(1). Nature Publishing Group
Nature Communications, 12(1). NATURE PORTFOLIO
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Allahyar, A, Pieterse, M, Swennenhuis, J, Los-de Vries, G T, Yilmaz, M, Leguit, R, Meijers, R W J, van der Geize, R, Vermaat, J, Cleven, A, van Wezel, T, Diepstra, A, van Kempen, L O C, Hijmering, N J, Stathi, P, Sharma, M, Melquiond, A S J, de Vree, P J P, Verstegen, M J A M, Krijger, P H L, Hajo, K, Simonis, M, Rakszewska, A, van Min, M, de Jong, D, Ylstra, B, Feitsma, H, Splinter, E & de Laat, W 2021, ' Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing ', Nature Communications, vol. 12, no. 1, 3361 . https://doi.org/10.1038/s41467-021-23695-8

In routine diagnostic pathology, cancer biopsies are preserved by formalin-fixed, paraffin-embedding (FFPE) procedures for examination of (intra-) cellular morphology. Such procedures inadvertently induce DNA fragmentation, which compromises sequenci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f015170cc76e934c99a06da52084f3ba
https://research.vumc.nl/en/publications/dbd7c41c-2950-4947-b268-32207673d8fd

Biosynthesis of a steroid metabolite by an engineered Rhodococcus erythropolis strain expressing a mutant cytochrome P450 BM3 enzyme

Autor: Kamila Z. Rosloniec, Evelien M Te Poele, Jan N. M. Commandeur, Nico P. E. Vermeulen, Robert van der Geize, Harini Venkataraman, Lubbert Dijkhuizen

Publikováno v: Venkataraman, H, te Poele, E M, Rosłoniec, K Z, Vermeulen, N, Commandeur, J N M, van der Geize, R & Dijkhuizen, L 2015, ' Biosynthesis of a steroid metabolite by an engineered Rhodococcus erythropolis strain expressing a mutant cytochrome P450 BM3 enzyme ', Applied Microbiology and Biotechnology, vol. 99, no. 11, pp. 4713-4721 . https://doi.org/10.1007/s00253-014-6281-7
Applied Microbiology and Biotechnology, 99(11), 4713-4721. SPRINGER
Applied Microbiology and Biotechnology, 99(11), 4713-4721. Springer Verlag

In the present study, the use of Rhodococcus erythropolis mutant strain RG9 expressing the cytochrome P450 BM3 mutant M02 enzyme has been evaluated for whole-cell biotransformation of a 17-ketosteroid, norandrostenedione, as a model substrate. Purifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bab8cbaa5413f9cc885911cb629e81c
http://www.scopus.com/inward/record.url?scp=84939964784&partnerID=8YFLogxK

Molecular characterization of three 3-ketosteroid-Delta(1)-dehydrogenase isoenzynnes of Rhodococcus ruber strain Chol-4

Autor: Robert van der Geize, Juana María Navarro Llorens, Julián Perera, Laura Fernández de las Heras, Oliver Drzyzga

Publikováno v: Journal of steroid biochemistry and molecular biology, 132(3-5), 271-281. PERGAMON-ELSEVIER SCIENCE LTD

Rhodococcus ruber strain Chol-4 isolated from a sewage sludge sample is able to grow on minimal medium supplemented with steroids, showing a broad catabolic capacity. This paper reports the characterization of three different 3-ketosteroid-Delta(1)-d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa616d3b10432ed5cfb36ab6d0e98692
https://research.rug.nl/en/publications/5239cf3e-36f9-4cf4-923e-9f8104f00819