Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Robert W. Rubin"'
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 89(4)
Rhythmic, spontaneously pulsating cardiac cells cultured from newborn rats are immediately stimulated to beat faster by addition of a number of tubulin-binding agents but not by their non-tubulin-binding analogues. The tubulin-binding agents tested i
Publikováno v:
American Zoologist. 16:547-561
An analysis of the changing patterns of protein synthesis during early development of Lymnaea palustris has been undertaken. An examination of the ingestion of capsule fluid protein suggests that after gastrulation 30 to 65% of the total embryo prote
Autor:
Robert W. Rubin, Mary A. Bonneville, Ross Wilkins, William P. Cunningham, L. Andrew Staehelin
Publikováno v:
The Journal of Cell Biology
Isolated Golgi complexes can be recognized in phosphotungstate (PTA) negative stain as stacks of membranous plates surrounded by a complex anastomosing network of tubules and vesicles. The extent of this tubular network is, however, much greater than
Publikováno v:
Cell Biology International Reports. 6:177-187
Plasma membranes were isolated by adhering normal rat kidney cells (NRK) and their Kirsten viral transformants (442 cells) to a poly-L-lysine treated surface, and lysing them with hypotonic buffer. Scanning and transmission electron micrographs of su
Publikováno v:
Tissue and Cell. 8:209-215
A method is described for the quantitation of the pool size of a tubulin like protein during synchronized cell division in the sea urchin Lytechinus variegatus. The method involves the use of a thin SDS slab polyacrylamide gel system in which tubulin
Publikováno v:
The Journal of Cell Biology
The amount of actin and total protein per cell in normal rat kidney (NRK) cells in culture is initially high in very low density cultures, but rapidly decreases as the cells come into contact in higher density cultures. In a viral transformant of NRK
Autor:
Julie R. Craik, Kermit L. Carraway, Coralie A. Carothers Carraway, Goeh Jung, Robert W. Rubin
Publikováno v:
Experimental Cell Research. 143:303-308
Microvilli isolated from MAT-C1 13762 ascites tumor cells were extracted with Triton X-100 in phosphate-buffered saline (PBS) to yield cytoskeletal residues. Analysis of the residues by two-dimensional isoelectric focusing-dodecyl sulfate electrophor
Autor:
M. Maher, Robert W. Rubin
Publikováno v:
Experimental Cell Research. 103:159-168
A thin-slab, SDS polyacrylamide gel electrophoresis system is described in which actin within whole cell homogenates can be quantitated within a wide range of protein values (0.05–1.4 μg/band). After demonstrating the absence of appreciable contam
Autor:
Leighton P. Everhart, Robert W. Rubin
Publikováno v:
Journal of Cell Biology. 60:442-447
Cytochalasin B (CB) shows a marked concentration-dependent inhibition of the incorporation of [3H]thymidine into Chinese hamster ovary cells. This inhibition was shown to result from an inhibition of thymidine uptake, not from an inhibition of DNA sy
Autor:
Clara Milikowski, Robert W. Rubin
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 509:100-110
A modification of O'Farrell's method of two-dimensional polyacrylamide gel electrophoresis has allowed for the resolution of erythrocyte membranes showing up to 200 individual components. Data is presented which indicates that this protein heterogene