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pro vyhledávání: '"Robert Taft"'
Publikováno v:
Journal of Cardiac Failure. 29:595
Autor:
Robert Taft
Publikováno v:
Cold Spring Harbor protocols. 2017(11)
In vitro fertilization (IVF) involves fertilization of mature oocytes with capacitated sperm in a tissue culture dish. This technique can generate large numbers of cleavage-stage embryos without using a significant number of single-caged stud males f
Autor:
Robert Taft
Publikováno v:
Cold Spring Harbor protocols. 2017(11)
Significant progress has been made in sperm cryopreservation. It is now possible to achieve more consistent fertilization rates using in vitro fertilization (IVF) with cryorecovered sperm. Including the antioxidant monothioglycerol (MTG) in the cryop
Publikováno v:
Methods in Molecular Biology ISBN: 9781493936595
Highly definable genetically, the humble mouse is the "reagent" mammal of choice with which to probe and begin to understand the human condition in all its complexities. With the recent advance in direct genome editing via targeted nucleases, e.g., T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a28906d055a2ae9826af5e930abcb5e
https://doi.org/10.1007/978-1-4939-3661-8_3
https://doi.org/10.1007/978-1-4939-3661-8_3
Autor:
Robert Taft
Publikováno v:
Cold Spring Harbor Protocols. 2018:pdb.prot094540
This embryo cryopreservation protocol is used by The Jackson Laboratory and several other repositories around the world. This protocol has clearly withstood the test of time; >2 million embryos from thousands of strains have been cryopreserved using
Autor:
Robert Taft, Muriel T. Davisson
Publikováno v:
Brain Research. 1091:255-257
Over 10,000 mouse strains and more than 20,000 mouse ES cells containing gene-trapped or targeted mutations are available to researchers. The mouse strains and stocks include those with spontaneous, genetically engineered and induced mutations, as we
Autor:
Stephen A. Murray, Robert Taft, Shannon L. Byers, Peter M. Kutny, Benjamin E. Low, Michael V. Wiles
Publikováno v:
PLoS ONE, Vol 8, Iss 7, p e67826 (2013)
PLoS ONE
PLoS ONE
There is a continual need to improve efficiency in creating precise genetic modifications in mice using embryonic stem cells (ESCs). We describe a novel approach resulting in 100% germline transmission from competent injected ESCs. We developed an F1
Publikováno v:
PLoS ONE
PLoS ONE, Vol 7, Iss 4, p e35538 (2012)
PLoS ONE, Vol 7, Iss 4, p e35538 (2012)
The efficiency of producing timed pregnant or pseudopregnant mice can be increased by identifying those in proestrus or estrus. Visual observation of the vagina is the quickest method, requires no special equipment, and is best used when only proestr
Autor:
Eric, Antoniou, Robert, Taft
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 825
Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not pos
Autor:
Robert Taft, Eric Antoniou
Publikováno v:
Methods in Molecular Biology ISBN: 9781617794353
Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not pos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::33179c859fa7e3bb23851d3949ccdaa3
https://doi.org/10.1007/978-1-61779-436-0_18
https://doi.org/10.1007/978-1-61779-436-0_18