Výsledky vyhledávání - "Robert T. Filla"

LC-MS/MS quantitation of insulin, glucagon, somatostatin, and C-peptide v1

Autor: Matthew J. Donohue, Robert T. Filla, Daniel J. Steyer, Wesley J. Eaton, Michael G. Roper

An LC-MS method using multiple reaction monitoring for quantitation of insulin, C-peptide, glucagon, and somatostatin secretion from human islet populations is described. A 5 min separation was developed using a 2.1 × 30 mm (i.d. x length) C18 colum

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::286c51111f8c8b8920b0b1082f5e466a
https://doi.org/10.17504/protocols.io.buw9nxh6

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Determination of online quenching efficiency for an automated cellular microfluidic metabolomic platform using mass spectrometry based ATP degradation analysis

Autor: Laura A. Filla, Katherine L. Sanders, Robert T. Filla, John B Coulton, James L. Edwards

Publikováno v: Analytical and bioanalytical chemistry. 411(24)

As microfluidic cell culture progresses, the need for robust and reproducible intracellular analyses grows. In particular, intracellular metabolites are subject to perturbation and degradation during the lysing process. The reliability of intracellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbb07597ef6587b37d4bbf3b04379bb5
https://pubmed.ncbi.nlm.nih.gov/31372700

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Rapid liquid chromatography-mass spectrometry quantitation of glucose-regulating hormones from human islets of Langerhans

Autor: Michael G. Roper, Wesley J. Eaton, Robert T. Filla, Matthew J. Donohue, Daniel J. Steyer

Publikováno v: J Chromatogr A

Glucose homeostasis is maintained through the secretion of peptide hormones, such as insulin, somatostatin, and glucagon, from islets of Langerhans, clusters of endocrine cells found in the pancreas. This report describes an LC-MS method using multip

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::708f6dbcdc125db8c2da38dc5f5bae75
https://doi.org/10.1016/j.chroma.2020.461805

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Frequency-Modulated Continuous Flow Analysis Electrospray Ionization Mass Spectrometry (FM-CFA-ESI-MS) for Sample Multiplexing

Autor: Adrian M. Schrell, James L. Edwards, John B Coulton, Robert T. Filla, Michael G. Roper

Publikováno v: Analytical chemistry. 90(4)

A method for multiplexed sample analysis by mass spectrometry without the need for chemical tagging is presented. In this new method, each sample is pulsed at unique frequencies, mixed, and delivered to the mass spectrometer while maintaining a const

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bafc3c8f67e17e30eca7b199e92ae060
https://pubmed.ncbi.nlm.nih.gov/29356503

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Automated Sample Preparation in a Microfluidic Culture Device for Cellular Metabolomics

Autor: Laura A. Filla, Robert T. Filla, James L. Edwards, Katherine L. Sanders

Sample pretreatment in conventional cellular metabolomics entails rigorous lysis and extraction steps which increase the duration as well as limit the consistency of these experiments. We report a biomimetic cell culture microfluidic device (MFD) whi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65a422335864ec875a70e5c77d1e2967
https://europepmc.org/articles/PMC4902300/

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