Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Robert Steven Siegel"'
Publikováno v:
Amyloid. 3:100-109
A characteristic neuropathologic feature of Alzheimer c disease is an abundance of brain-localized neuritic plaques. These plaques are identified as areas of degenerating nerve terminals surrounding cores of aggregated and insoluble fibrils of amyloi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::12e519ea44304db13e746e721ecace98
https://doi.org/10.3109/13506129609014361
https://doi.org/10.3109/13506129609014361
Autor:
Thomas S. Vedvick, Michael E. Engel, Gregory Patrick Thill, Richard Gordon Buckholz, Robert Steven Siegel, Jennifer Anne Kinney, Sally Provow, Mary Susan Urcan
Publikováno v:
Journal of Industrial Microbiology. 7:197-201
A synthetic gene encoding aprotinin (bovine pancreatic trypsin inhibitor) was fused to the Saccharomyces cerevisiae prepro alpha mating factor leader sequence at the dibasic amino acid processing site. Pichia pastoris strains were developed to expres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7848bfc05acbd63e71e5c778606094ba
https://doi.org/10.1007/bf01575883
https://doi.org/10.1007/bf01575883
Autor:
Barry R. Neiditch, Steven L. Wagner, Robert Steven Siegel, Alvin H. Schmaier, William E. Van Nostrand, William C. Raschke, Sangram S. Sisodia
Publikováno v:
Biochimica et biophysica acta. 1209(2)
In this report we describe the use of the methylotrophic industrial yeast Pichia pastoris as a host system for the large scale production of the Kunitz-type proteinase inhibitor (KPI) domain of the amyloid beta-protein precursor-like protein-2 (APLP-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bc92c22da8aa2e4bb73210d98ffadeb
https://pubmed.ncbi.nlm.nih.gov/7811686
https://pubmed.ncbi.nlm.nih.gov/7811686
Autor:
Robert Steven Siegel, William E. Van Nostrand, Thomas S. Vedvick, William C. Raschke, Steven L. Wagner
Publikováno v:
Biochemical and biophysical research communications. 186(2)
The protease inhibitor, protease nexin-2 (PN-2), is the secreted isoform of the Alzheimer's amyloid beta-protein precursor (A beta PP) that contains the Kunitz-type protease inhibitor (KPI) domain. Here we describe the use of the methylotrophic indus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1675172e8b70585ba90beb6384acb68b
https://pubmed.ncbi.nlm.nih.gov/1497647
https://pubmed.ncbi.nlm.nih.gov/1497647
Autor:
William Scot Craig, Robert Steven Siegel, Thomas F. Mowles, Saraswathi Patthi, Gregory Patrick Thill, Geneva Ruth Davis, Gonul Velicelebi, Lillian Margaret Wondrack
Publikováno v:
International journal of peptide and protein research. 38(5)
An analog of growth hormone releasing factor (GRF), [Leu27]GRF(1-40)-OH, has been expressed and secreted in Saccharomyces cerevisiae under the control of the alpha-factor gene promoter and prepro sequence. A single pair of consecutive basic residues
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83dea790ebe137c09b7e66f185345611
https://pubmed.ncbi.nlm.nih.gov/1802859
https://pubmed.ncbi.nlm.nih.gov/1802859
Publikováno v:
Bio/technology (Nature Publishing Company). 8(7)
Operation of a continuous microbial fermentor with cell recycle can significantly reduce degradation-associated loss of a secreted protein product. Under continuous fermentation conditions, proteolysis of a recombinant growth hormone releasing factor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2727944d97bba59833cea2613aedc5ae
https://pubmed.ncbi.nlm.nih.gov/1366627
https://pubmed.ncbi.nlm.nih.gov/1366627
Autor:
Christopher Michael Bussineau, Robert Steven Siegel, Russell Arthur Brierley, A. Melton, R. Kosson
Publikováno v:
Annals of the New York Academy of Sciences. 589
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51c2e4d3d01fe205cd16d8e6742b149c
https://pubmed.ncbi.nlm.nih.gov/2357028
https://pubmed.ncbi.nlm.nih.gov/2357028
Autor:
William Scot Craig, B. L. Smiley, Knut Madden, Robert Steven Siegel, Cathy A. Stillman, G. Velic ecedil, James M. Cregg, Richard Gordon Buckholz, J. Cruze, Juerg Friedrich Tschopp, P. A. Kellaris, Gregory Patrick Thill, Geneva Ruth Davis, M. Akong, R. Torregrossa, lebi
Publikováno v:
Nature Biotechnology. 5:479-485
The methylotrophic industrial yeast, Pi–chia pastoris, has been developed as a host system for the large–scale production of heterologous proteins. As an example of the systems, the synthesis of hepatitis B surface antigen (HBsAg) is described. E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::08dcc66fa53b10bd7b517702a4b7efa7
https://doi.org/10.1038/nbt0587-479
https://doi.org/10.1038/nbt0587-479