CRISPR/Cas9 targeted inactivation of the kauniolide synthase in chicory results in accumulation of costunolide and its conjugates in taproots

Autor: Katarina Cankar, Johanna Christina Hakkert, Robert Sevenier, Eva Campo, Bert Schipper, Christina Papastolopoulou, Khabat Vahabi, Alain Tissier, Paul Bundock, Dirk Bosch

Publikováno v: Frontiers in Plant Science, Vol 13 (2022)

Chicory taproots accumulate sesquiterpene lactones lactucin, lactucopicrin, and 8-deoxylactucin, predominantly in their oxalated forms. The biosynthetic pathway for chicory sesquiterpene lactones has only partly been elucidated; the enzymes that conv

Externí odkaz: https://doaj.org/article/5263b58d5b36440aa73967f92ec132c3

Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus L

Autor: Ingrid M. van der Meer, Jules Beekwilder, Suvi T. Häkkinen, Johanna C. Hakkert, Dirk Bosch, Katarina Cankar, Robert Sevenier, Michiel Theodoor Jan De Both, Paul Bundock

Publikováno v: Plant Biotechnology Journal
Cankar, K, Bundock, P, Sevenier, R, Häkkinen, S T, Hakkert, J C, Beekwilder, J, van der Meer, I M, de Both, M & Bosch, D 2021, ' Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus L. ', Plant Biotechnology Journal, vol. 19, no. 12, pp. 2442-2453 . https://doi.org/10.1111/pbi.13670
Plant Biotechnology Journal, 19(12), 2442-2453
Plant Biotechnology Journal 19 (2021) 12

Chicory (Cichorium intybus var. sativum) is an industrial crop species cultivated for the production of a fructose polymer inulin, which is used as a low‐calorie sweetener and prebiotic. Besides, inulin chicory taproots also accumulate sesquiterpen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c377b14acd732a24f3a04f2ace32680
https://cris.vtt.fi/en/publications/510f9619-8b84-4a7c-9466-c1f9741a1fe9

Gain and loss of fruit flavour compounds produced by wild and cultivated strawberry species

Autor: Zhongkui Sun, Asaph Aharoni, Wilfried Schwab, Robert Sevenier, Ashok P. Giri, Maarten A. Jongsma, Francel W.A. Verstappen, Cinzia M. Bertea, Harro J. Bouwmeester

Publikováno v: The Plant Cell, 16, 3110-3131
The Plant Cell 16 (2004)

The blends of flavor compounds produced by fruits serve as biological perfumes used to attract living creatures, including humans. They include hundreds of metabolites and vary in their characteristic fruit flavor composition. The molecular mechanism

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d13e0829e96c911675673355465d256
https://doi.org/10.1105/tpc.104.023895

Increased production of nutriments by genetically engineered crops

Autor: Ingrid M. van der Meer, Raoul Bino, A.J. Koops, Robert Sevenier

Publikováno v: Journal of the American College of Nutrition, 21(3S), 199S-204S
Journal of the American College of Nutrition 21 (2002) 3S

Plants are the basis of human nutrition and have been selected and improved to assure this purpose. Nowadays, new technologies such as genetic engineering and genomics approaches allow further improvement of plants. We describe here three examples fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c65a108b7c05ea759806b753790e97f3
https://research.wur.nl/en/publications/increased-production-of-nutriments-by-genetically-engineered-crop

CRISPR/Cas9 targeted inactivation of the kauniolide synthase in chicory results in accumulation of costunolide and its conjugates in taproots

Autor: Katarina Cankar, Johanna Christina Hakkert, Robert Sevenier, Eva Campo, Bert Schipper, Christina Papastolopoulou, Khabat Vahabi, Alain Tissier, Paul Bundock, Dirk Bosch

Publikováno v: Frontiers in Plant Science 13 (2022)
Frontiers in Plant Science, 13

Chicory taproots accumulate sesquiterpene lactones lactucin, lactucopicrin, and 8-deoxylactucin, predominantly in their oxalated forms. The biosynthetic pathway for chicory sesquiterpene lactones has only partly been elucidated; the enzymes that conv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d48009f883f8ab004785e93f38a17a2