Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Robert Sarfati"'
Autor:
Karine Barral, Karine Alvarez, Boulbaba Selmi, Laurence A. Mulard, Bruno Canard, Catherine Guerreiro, Joëlle Boretto, Robert Sarfati, Jerome Deval
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids
Nucleosides, Nucleotides and Nucleic Acids, Taylor & Francis, 2005, 24 (5-7), pp.419-422. ⟨10.1081/NCN-200059951⟩
Nucleosides, Nucleotides and Nucleic Acids, 2005, 24 (5-7), pp.419-422. ⟨10.1081/NCN-200059951⟩
Nucleosides, Nucleotides and Nucleic Acids, Taylor & Francis, 2005, 24 (5-7), pp.419-422. ⟨10.1081/NCN-200059951⟩
Nucleosides, Nucleotides and Nucleic Acids, 2005, 24 (5-7), pp.419-422. ⟨10.1081/NCN-200059951⟩
International audience; Alpha-boranophosphates suppress RT-mediated resistance when the catalytic rate of incorporation (kpol) of the analogue 5'-triphosphate is responsable for drug resistance, such as in the case of K65R mutant and ddNTPs, and Q151
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2fa8adee3bd8b1430548b6cff548dc9
https://doi.org/10.1081/ncn-200059951
https://doi.org/10.1081/ncn-200059951
Publikováno v:
Journal of Bioenergetics and Biomembranes. 32:317-324
Nucleoside analogs are currently used in antiretrovirus therapies. The best known example isAZT one of the first drug to be used for the treatment of AIDS. However, only the triphosphatederivatives of these compounds act as substrates of the viral re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c758ec564bd2cc1bc7ceedb8b05ed0ef
https://doi.org/10.1023/a:1005501432684
https://doi.org/10.1023/a:1005501432684
Autor:
Michel Véron, Dominique Deville-Bonne, Ying Wu Xu, Joël Janin, Benoit Schneider, Robert Sarfati, Olivier Sellam
Publikováno v:
Journal of Biological Chemistry. 273:11491-11497
The pre-steady-state reaction ofDictyostelium nucleoside diphosphate (NDP) kinase with dideoxynucleotide triphosphates (ddNTP) and AZT triphosphate was studied by quenching of protein fluorescence after manual mixing or by stopped flow. The fluoresce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b365f17bfea61b7c91b92e80b521b5f
https://doi.org/10.1074/jbc.273.19.11491
https://doi.org/10.1074/jbc.273.19.11491
Publikováno v:
Proceedings of the National Academy of Sciences. 94:11279-11284
HIV-1 reverse transcriptase (RT) catalyzes the synthesis of DNA from DNA or RNA templates. During this process, it must transfer its primer from one template to another RNA or DNA template. Binary complexes made of RT and a primer/template bind an ad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32e4d401f45e27b1669a47ec43a794c4
https://doi.org/10.1073/pnas.94.21.11279
https://doi.org/10.1073/pnas.94.21.11279
Publikováno v:
Nucleosides and Nucleotides. 16:1757-1760
Dibenzyl-3′-O-[(6-azido-2,3,6-trideoxy-4,5-di-O-benzyl) hexanoyl] thymidine 5′-yl phosphate 8a was prepared. Catalytic hydrogenolysis removed only the benzyl esters and reduced the azido group. When the benzyl ethers were replaced by p-phenylbenz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1dc830a829e8d9bac89a0f54efdbc2d9
https://doi.org/10.1080/07328319708006271
https://doi.org/10.1080/07328319708006271
Publikováno v:
Proceedings of the National Academy of Sciences. 92:10859-10863
Enzymatic incorporation of 2',3'-dideoxynucleotides into DNA results in chain termination. We report that 3'-esterified 2'-deoxynucleoside 5'-triphosphates (dNTPs) are false chain-terminator substrates since DNA polymerases, including human immunodef
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46a2afe689c3f9490a5de16ee77710db
https://doi.org/10.1073/pnas.92.24.10859
https://doi.org/10.1073/pnas.92.24.10859
Autor:
Constantin T. Craescu, Nicolae Palibroda, H. Sakamoto, Robert Sarfati, M. Chiriac, O. Barzu, J. M. Smith, Ahmed Bouhss
Publikováno v:
Analytical Biochemistry. 225:18-23
GMP and dGMP labeled with 15N at the 2-amino group of the purine ring was obtained enzymatically from NH4Cl (>99 at.% 15N) and from IMP or dIMP, respectively, by several reactions involving IMP-dehydrogenase, GMP-synthetase, adenylate kinase, and cre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a6547e2584015cdb8446be03726db8d
https://doi.org/10.1006/abio.1995.1101
https://doi.org/10.1006/abio.1995.1101
Publikováno v:
J. Chem. Soc., Perkin Trans. 1. :1163-1171
Treatment, of 5′-O-dimethoxytritylthymidine 1 and of 4-N-benzyloxycarbonyl-2′-deoxy-5′-O-dimethoxy-trityl-cytidine 12 with a mixture of 6-(benzyloxycarbonylamino)hexanoic acid, dicyclohexylcarbodiimide and 4-(dimethylamino)pyridine yielded 3′
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6aea1fae701199ba0499dd7d45ad6a5
https://doi.org/10.1039/p19950001163
https://doi.org/10.1039/p19950001163
Autor:
Bruno Canard, Robert Sarfati
Publikováno v:
Gene. 148:1-6
We have synthesized 3'-substituted-2'-deoxyribonucleotide-5'-triphosphates corresponding to A, T, G and C. The 3' position was esterified by a separate anthranylic derivative (3'-tag) giving specific fluorescent properties to each nucleotide (nt). Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe7aae60319e30f223d8469c7f4c1a46
https://doi.org/10.1016/0378-1119(94)90226-7
https://doi.org/10.1016/0378-1119(94)90226-7
Publikováno v:
FEBS Letters. 330:334-338
This work studies the structure-function relationships of Asn135, a residue situated in the GTP binding pocket of elongation factor Tu (EF-Tu). For this purpose we constructed EF-TuN135D/D138N and assayed its reactivity towards various purine nucleot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85c5dfffd164a4bf682abad1f0f21855
https://doi.org/10.1016/0014-5793(93)80899-6
https://doi.org/10.1016/0014-5793(93)80899-6