B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation

Autor: Benjamin G. Barwick, Christopher D. Scharer, Ryan J. Martinez, Madeline J. Price, Alexander N. Wein, Robert R. Haines, Alexander P. R. Bally, Jacob E. Kohlmeier, Jeremy M. Boss

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)

DNA methylation is known to contribute to B cell differentiation, but de novo methylation has not been studied in this context. Here the authors use a conditional Dnmt3a/b knockout mouse to map the function of de novo DNA methylation in B cell differ

Externí odkaz: https://doaj.org/article/33af14121bb04d5cbdd45624b424b0e7

The Histone Demethylase LSD1 Regulates B Cell Proliferation and Plasmablast Differentiation

Autor: Robert R. Haines, Christopher D. Scharer, Parimal Majumder, Troy D. Randall, Benjamin G. Barwick, Jeremy M. Boss

Publikováno v: Journal of immunology (Baltimore, Md. : 1950). 201(9)

B cells undergo epigenetic remodeling as they differentiate into antibody-secreting cells. LSD1 is a histone demethylase known to decommission active enhancers and cooperate with the antibody-secreting cell master regulatory transcription factor Blim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35bc38e3f20a0fff3875d30ab22d52ac
https://pubmed.ncbi.nlm.nih.gov/30232138

EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production

Autor: Anna K. Kania, Dillon G. Patterson, Madeline J. Price, Troy D. Randall, Robert R. Haines, Muyao Guo, Jeremy M. Boss, Benjamin G. Barwick, John E. Bradley, Christopher D. Scharer

Publikováno v: Journal of immunology (Baltimore, Md. : 1950). 200(3)

Epigenetic remodeling is required during B cell differentiation. However, little is known about the direct functions of epigenetic enzymes in Ab-secreting cells (ASC) in vivo. In this study, we examined ASC differentiation independent of T cell help

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c42a2cd821d51a67cd0c47e854fa8fc
https://pubmed.ncbi.nlm.nih.gov/29288200

ATAC-seq on biobanked specimens defines a unique chromatin accessibility structure in naïve SLE B cells

Autor: Christopher D. Scharer, Chungwen Wei, Benjamin G. Barwick, Ignacio Sanz, Robert R. Haines, Jeremy M. Boss, Emily L. Blalock

Publikováno v: Scientific Reports

Biobanking is a widespread practice for storing biological samples for future studies ranging from genotyping to RNA analysis. However, methods that probe the status of the epigenome are lacking. Here, the framework for applying the Assay for Transpo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81bab51e18f24a955015414d81e47c12

Regional protein expression in human Alzheimer's brain correlates with disease severity.

Autor: Xu J; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK.; 2School of Biological Sciences, and Maurice Wilkins Centre for Molecular Biodiscovery, Faculty of Science, University of Auckland, Private Bag 92019, Auckland, 1142 New Zealand., Patassini S; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK.; 2School of Biological Sciences, and Maurice Wilkins Centre for Molecular Biodiscovery, Faculty of Science, University of Auckland, Private Bag 92019, Auckland, 1142 New Zealand., Rustogi N; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK., Riba-Garcia I; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK., Hale BD; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK., Phillips AM; 3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, L69 3GJ UK., Waldvogel H; 4Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142 New Zealand., Haines R; 5Research IT, The University of Manchester, Manchester, M13 9PL UK., Bradbury P; 5Research IT, The University of Manchester, Manchester, M13 9PL UK., Stevens A; 6Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, M13 9PL UK., Faull RLM; 4Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142 New Zealand., Dowsey AW; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK.; 7Department of Population Health Sciences and Bristol Veterinary School, Faculty of Health Sciences, University of Bristol, Bristol, BS8 2BN UK., Cooper GJS; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK.; 2School of Biological Sciences, and Maurice Wilkins Centre for Molecular Biodiscovery, Faculty of Science, University of Auckland, Private Bag 92019, Auckland, 1142 New Zealand.; 4Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142 New Zealand., Unwin RD; 1Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Sciences Centre, Core Technology Facility (3rd Floor), 46 Grafton Street, Manchester, M13 9NT UK.

Publikováno v: Communications biology [Commun Biol] 2019 Feb 04; Vol. 2, pp. 43. Date of Electronic Publication: 2019 Feb 04 (Print Publication: 2019).