Accidental Encounter of Repair Intermediates in Alkylated DNA May Lead to Double-Strand Breaks in Resting Cells

Autor: Shingo Fujii, Robert P. Fuchs

Publikováno v: International Journal of Molecular Sciences, Vol 25, Iss 15, p 8192 (2024)

In clinics, chemotherapy is often combined with surgery and radiation to increase the chances of curing cancers. In the case of glioblastoma (GBM), patients are treated with a combination of radiotherapy and TMZ over several weeks. Despite its common

Externí odkaz: https://doaj.org/article/bff9d94038a843c5b8626c945674587d

Enrichment of plasmid pool from E. coli for mass sequencing to detect untargeted mutagenic events

Autor: Asako Isogawa, Robert P. Fuchs, Shingo Fujii

Publikováno v: STAR Protocols, Vol 2, Iss 2, Pp 100399- (2021)

Summary: Translesion synthesis (TLS) is an event to cope with DNA damages. During TLS, the responsible TLS polymerase frequently elicits untargeted mutagenesis as potentially a source of genetic diversity. Identifying such untargeted mutations in viv

Externí odkaz: https://doaj.org/article/ebd7338c665b4d328ac104b470d4ba0c

Identification of Proteins Specifically Assembled on a Stem-Loop Composed of a CAG Triplet Repeat

Autor: Fujii, Robert P. Fuchs, Asako Isogawa, Joao A. Paulo, Shingo

Publikováno v: DNA; Volume 3; Issue 2; Pages: 109-118

Human genomic DNA contains a number of diverse repetitive sequence motifs, often identified as fragile sites leading to genetic instability. Among them, expansion events occurring at triplet repeats have been extensively studied due to their associat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=multidiscipl::4c2148097000212875f9e426b735fc05

Double-strand breaks: When DNA repair events accidentally meet

Autor: Shingo Fujii, Robert W. Sobol, Robert P. Fuchs

Publikováno v: DNA Repair
DNA Repair, 2022, 112, pp.103303. ⟨10.1016/j.dnarep.2022.103303⟩
DNA Repair, 2022, 112, ⟨10.1016/j.dnarep.2022.103303⟩
DNA Repair (Amst)

International audience; The cellular response to alkylation damage is complex, involving multiple DNA repair pathways and checkpoint proteins, depending on the DNA lesion, the cell type, and the cellular proliferation state. The repair of and respons

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::037485700e93b5ef509b318bc3087ac0
https://hal.science/hal-03813433/document

Crosstalk between repair pathways elicits double-strand breaks in alkylated DNA and implications for the action of temozolomide

Autor: Joao A. Paulo, Kazumitsu Onizuka, Shingo Fujii, Asako Isogawa, Robert P Fuchs, Julien P. Duxin, Ravindra Amunugama, Tatsuro S. Takahashi

Publikováno v: eLife
eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.69544⟩
eLife, 2021, 10, ⟨10.7554/eLife.69544⟩
eLife, Vol 10 (2021)
Fuchs, R P, Isogawa, A, Paulo, J A, Onizuka, K, Takahashi, T, Amunugama, R, Duxin, J P & Fujii, S 2021, ' Crosstalk between repair pathways elicits Double Strand Breaks in alkylated DNA and implications for the action of temozolomide ', eLife, vol. 10, e69544 . https://doi.org/10.7554/eLife.69544

Temozolomide (TMZ), a DNA methylating agent, is the primary chemotherapeutic drug used in glioblastoma treatment. TMZ induces mostly N-alkylation adducts (N7-methylguanine and N3-methyladenine) and some O6-methylguanine (O6mG) adducts. Current models

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a20ea5955c4ee02a7ff8f68f31f373cf
https://hal.archives-ouvertes.fr/hal-03373140