Orai channel C-terminal peptides are key modulators of STIM-Orai coupling and calcium signal generation

Autor: James H. Baraniak, Jr., Yandong Zhou, Robert M. Nwokonko, Michelle R. Jennette, Sarah A. Kazzaz, Jazmin M. Stenson, Abigale L. Whitsell, Youjun Wang, Mohamed Trebak, Donald L. Gill

Publikováno v: Cell Reports, Vol 35, Iss 13, Pp 109322- (2021)

Summary: Junctional coupling between endoplasmic reticulum (ER) Ca2+-sensor STIM proteins and plasma membrane (PM) Orai channels mediates Ca2+ signals in most cells. We reveal that PM-tethered, fluorescently tagged C-terminal M4x (fourth transmembran

Externí odkaz: https://doaj.org/article/75378ddc54284ca0b1b93435967b376c

The STIM1-binding site nexus remotely controls Orai1 channel gating

Autor: Yandong Zhou, Xiangyu Cai, Natalia A. Loktionova, Xianming Wang, Robert M. Nwokonko, Xizhuo Wang, Youjun Wang, Brad S. Rothberg, Mohamed Trebak, Donald L. Gill

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)

How plasma membrane Orai Ca2+ channels are activated by STIM proteins to activate Ca2+signals is still not fully known. Here the authors show that a nexus region located at the Orai1 C-terminus allows channel gating without a direct interaction of ST

Externí odkaz: https://doaj.org/article/319437aa2a59462e85ad73d129f7e2ee

The oxytocin signaling complex reveals a molecular switch for cation dependence

Autor: Justin G. Meyerowitz, Michael J. Robertson, Ximena Barros-Álvarez, Ouliana Panova, Robert M. Nwokonko, Yang Gao, Georgios Skiniotis

Publikováno v: Nature Structural & Molecular Biology. 29:274-281

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ed88b0e4db33fb2250abf67e91861a76
https://doi.org/10.1038/s41594-022-00728-4

L-type Ca 2+ channel blockers promote vascular remodeling through activation of STIM proteins

Autor: Aparna Gudlur, Robert M. Nwokonko, Ryan E. Yoast, Mohamed Trebak, Nadine Hempel, Donald L. Gill, Martin Johnson, Scott M. Emrich, Ping Xin, Xuexin Zhang, Patrick G. Hogan, Raphael Jean Courjaret, Khaled Machaca, Wei Li

Publikováno v: Proceedings of the National Academy of Sciences. 117:17369-17380

Voltage-gated L-type Ca2+ channel (Cav1.2) blockers (LCCBs) are major drugs for treating hypertension, the preeminent risk factor for heart failure. Vascular smooth muscle cell (VSMC) remodeling is a pathological hallmark of chronic hypertension. VSM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5c41bb9e7240902f70e858f7fce582cb
https://doi.org/10.1073/pnas.2007598117

G-protein activation by a metabotropic glutamate receptor

Autor: Georgios Skiniotis, Marine de Lapeyrière, Jean-Philippe Rocher, Alpay B. Seven, Brian K. Kobilka, Justin G. Meyerowitz, Makaía M. Papasergi-Scott, Yang Gao, Ximena Barros-Álvarez, Jesper Mosolff Mathiesen, Robert M Nwokonko, Dominik Schelshorn, Michael J. Robertson, Chensong Zhang

Publikováno v: Nature
Seven, A B, Barros-Álvarez, X, de Lapeyrière, M, Papasergi-Scott, M M, Robertson, M J, Zhang, C, Nwokonko, R M, Gao, Y, Meyerowitz, J G, Rocher, J P, Schelshorn, D, Kobilka, B K, Mathiesen, J M & Skiniotis, G 2021, ' G-protein activation by a metabotropic glutamate receptor ', Nature, vol. 595, pp. 450-454 . https://doi.org/10.1038/s41586-021-03680-3

Family C G-protein-coupled receptors (GPCRs) operate as obligate dimers with extracellular domains that recognize small ligands, leading to G-protein activation on the transmembrane (TM) domains of these receptors by an unknown mechanism1. Here we sh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2b8806cda81f586e7d372c77289eeec
https://europepmc.org/articles/PMC8822903/