Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Robert M Hnasko"'
Publikováno v:
PLoS ONE, Vol 15, Iss 4, p e0231781 (2020)
The mushroom poison that causes the most deaths is the class of toxins known as amatoxins. Current methods to sensitively and selectively detect these toxins are limited by the need for expensive equipment, or they lack accuracy due to cross-reactivi
Externí odkaz:
https://doaj.org/article/0f7de6592ea441eabfc715503fa9b8a4
Autor:
Cathrin E Bruederle, Robert M Hnasko, Thomas Kraemer, Rafael A Garcia, Michael J Haas, William N Marmer, John Mark Carter
Publikováno v:
PLoS ONE, Vol 3, Iss 8, p e2969 (2008)
The epidemic of bovine spongiform encephalopathy (BSE) has led to a world-wide drop in the market for beef by-products, such as Meat-and-Bone Meal (MBM), a fat-containing but mainly proteinaceaous product traditionally used as an animal feed suppleme
Externí odkaz:
https://doaj.org/article/9ce3f1dd2abd48cdb1599304be286931
Publikováno v:
Toxins, Vol 11, Iss 7, p 407 (2019)
Botulism is a devastating disease caused by botulinum neurotoxins (BoNTs) secreted primarily by Clostridium botulinum. Mouse bioassays without co-inoculation with antibodies are the standard method for the detection of BoNTs, but are not capable of d
Externí odkaz:
https://doaj.org/article/60d3c9c8db67460e986174b3203f09d9
Publikováno v:
Journal of Food Protection. 82:2148-2153
Foodborne illness associated with fresh, ready-to-eat produce continues to be a significant challenge to public health. In this study, we created a phyllosphere-associated lactic acid bacteria (PLAB) library and screened it via a high-throughput in v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bc16e72f660d51f88bb58b34c633e926
https://doi.org/10.4315/0362-028x.jfp-19-246
https://doi.org/10.4315/0362-028x.jfp-19-246
Autor:
Larry H, Stanker, Robert M, Hnasko
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
The sandwich immunoassay (sELISA) is an invaluable technique for concentrating, detecting, and quantifying target antigens. The two critical components required are a capture antibody and a detection antibody, each binding a different epitope on the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::91e55cfd78c672f0b3e6c5cebf9536ac
https://pubmed.ncbi.nlm.nih.gov/26160565
https://pubmed.ncbi.nlm.nih.gov/26160565
Autor:
Robert M, Hnasko
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
Immunoglobulins (Ig) or antibodies are powerful molecular recognition tools that can be used to identify minute quantities of a given target analyte. Their antigen-binding properties define both the sensitivity and selectivity of an immunoassay. Unde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7d01987b9764e5aac62eae55ba4039bd
https://pubmed.ncbi.nlm.nih.gov/26160559
https://pubmed.ncbi.nlm.nih.gov/26160559
Autor:
Robert M, Hnasko, Larry H, Stanker
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3d225e958f4d9819615cfa3ba5ddfcf8
https://pubmed.ncbi.nlm.nih.gov/26160560
https://pubmed.ncbi.nlm.nih.gov/26160560
Autor:
Thomas S, Hnasko, Robert M, Hnasko
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c99dad4ba88b7ab0331f24abd4257b7f
https://pubmed.ncbi.nlm.nih.gov/26160567
https://pubmed.ncbi.nlm.nih.gov/26160567
Autor:
Robert M, Hnasko
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
The bioconjugation of an antibody to an enzymatic reporter such as horseradish peroxidase (HRP) affords an effective mechanism by which immunoassay detection of a target antigen can be achieved. The use of heterobifunctional cross-linkers to covalent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::16e5ac68785784a0a731050003e913b8
https://pubmed.ncbi.nlm.nih.gov/26160562
https://pubmed.ncbi.nlm.nih.gov/26160562
Autor:
Robert M, Hnasko, Jeffery A, McGarvey
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1318
Antibodies are provided in a variety of formats that include antiserum, hybridoma culture supernatant, or ascites. They can all be used successfully in crude form for the detection of target antigens by immunoassay. However, it is advantageous to use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8ba853d7970d6be0433b60edb2a514a4
https://pubmed.ncbi.nlm.nih.gov/26160561
https://pubmed.ncbi.nlm.nih.gov/26160561