Zobrazeno 1 - 10
of 102
pro vyhledávání: '"Robert L. Metzenberg"'
Autor:
Robert L. Metzenberg, Nirmala Bardiya, Patrick K. T. Shiu, Patricia J. Pukkila, Edward G. Barry, William G. Alexander, Tony D. Perdue
Publikováno v:
Genetics. 178:593-596
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have succ
Publikováno v:
Genetics. 176:43-52
In Neurospora crassa, pairing of homologous DNA segments is monitored during meiotic prophase I. Any genes not paired with a homolog, as well as any paired homologs of that gene, are silenced during the sexual phase by a mechanism known as meiotic si
Autor:
Matthew G. Endrizzi, Li-Jun Ma, Ian T. Paulsen, Gregory O. Kothe, David B. Jaffe, David E. A. Catcheside, Edward M. Marcotte, Jonathan Butler, Giuseppe Macino, Matthew S. Sachs, David D. Perkins, Jerome Naylor, Oded Yarden, Nick O. Read, Shunguang Wang, Sarah E. Calvo, Reinhard Engels, J. Andrew Berglund, Nicole Stange-Thomann, Robert L. Metzenberg, Stephan Seiler, Scott Kroken, Seth Purcell, Dmitrij Frishman, Ulrich Schulte, Bruce W. Birren, Dayong Qui, Manolis Kamvysselis, Edward L. Braun, Gregory Jedd, Rodolfo Aramayo, Mary Anne Nelson, Sante Gnerre, Carlo Cogoni, Deborah Bell-Pedersen, Rodger B. Voelker, Chad Nusbaum, David Greenberg, Robert J. Pratt, Gertrud Mannhaupt, Bushra Rehman, Carolyn G. Rasmussen, Colin P.C. DeSouza, Michael Plamann, Weixi Li, Evan Mauceli, Daniel J. Ebbole, Katherine A. Borkovich, William Fitzhugh, Svetlana Krystofova, Peter Ianakiev, Claude P. Selitrennikoff, Stephen A. Osmani, Marc J. Orbach, Alice Roy, Jay C. Dunlap, Donald O. Natvig, Robert Barrett, Stephen Rudd, Louise Glass, James E. Galagan, Cord Bielke, Karen Foley, Alan Radford, John A. Kinsey, Michael Freitag, Chuck Staben, Lisa A. Alex, Alex Zelter, Cydney B. Nielsen, Margaret Werner-Washburne, Serge Smirnov, Timothy Elkins, Michael Kamal, Werner Mewes, Eric S. Lander, Eric U. Selker
Publikováno v:
Nature. 422:859-868
Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000
Publikováno v:
Cell. 107:905-916
The silencing of gene expression by segments of DNA present in excess of the normal number is called cosuppression in plants and quelling in fungi. We describe a related process, meiotic silencing by unpaired DNA (MSUD). DNA unpaired in meiosis cause
Publikováno v:
Molecular and General Genetics MGG. 259:615-621
Complexity in and around the A and a mating type idiomorphs in Neurospora crassa was examined. Six sets of transcripts surrounding the idiomorphs were identified by Northern analysis. Several different patterns of regulation were observed. The two pa
Autor:
Judith N. Stevens, Eric U. Selker, Brian S. Margolin, Phillip W. Garrett-Engele, Carrie Garrett-Engele, Robert L. Metzenberg, Deborah Y. Fritz
Publikováno v:
Genetics. 149:1787-1797
In an analysis of 22 of the roughly 100 dispersed 5S rRNA genes in Neurospora crassa, a methylated 5S rRNA pseudogene, Ψ63, was identified. We characterized the Ψ63 region to better understand the control and function of DNA methylation. The 120-bp
Autor:
Robert L. Metzenberg
Publikováno v:
Fungal Genetics Reports. 52:11-13
Publikováno v:
Genetics. 144:991-1003
This report describes the identification, cloning, and molecular analysis of Asm-1 + (Ascospore maturation 1), the Neurospora crassa homologue of the Aspergillus nidulans stuA (stunted A) gene. The Asm-1 + gene is constitutively transcribed and encod
Autor:
Wayne K. Versaw, Robert L. Metzenberg
Publikováno v:
Analytical Biochemistry. 241:14-17
To study, in vivo, potential P i –water oxygen exchange catalyzed by each of two high-affinity P i symporters of Neurospora crassa, we have developed methods for the purification of P i from whole-cell extracts and the subsequent derivatization of
Publikováno v:
Molecular and General Genetics MGG. 252:709-716
In response to phosphorus limitation, the fungus Neurospora crassa synthesizes a number of enzymes that function to bring more phosphate into the cell. The NUC-2 protein appears to sense the availability of phosphate and transmits the signal downstre